The R.E.A.L. Prep 96 procedure processes multiples of 96 samples in parallel using both vacuum-driven transfer and centrifugation. The procedure is based on a modified alkaline lysis followed by efficient filtration through a special filter unit, the QIAfilter 96.
Agarose gel analysis of shotgun clones from an S. pombe shotgun library purified using the R.E.A.L. Prep 96 procedure is shown. Approximately 200 ng DNA was loaded per lane onto a 1% TAE agarose gel. Outer lanes: markers
Phred 20 analysis of 11,808 plasmid shotgun sequences derived from a bacterial genome prepared with the R.E.A.L. Prep 96 System is shown. Samples were sequenced with BigDye Terminator sequencing chemistries (quarter reactions) and analyzed on an ABI PRISM 3700 DNA Analyzer with a POP-5 gel matrix. The analysis shows the percentage of sequencing reads, which were calculated using sequential windows of 10 bases.