High level of cell death.
High level of cell death.
Cell death in primary cells.
Cell death in primary cells.
Easy siRNA transfection optimization.
Easy siRNA transfection optimization.
High level of cell death. [A]  SW620 cells or  [B] SW480 cells (5 x 103 cells per well in 96-well plates) were transfected with 20 nM of AllStars Hs Cell Death Control siRNA or AllStars Negative Control siRNA or an siRNA targeting PLK (a well-known target for induction of cell death). Untransfected cells and cells treated with transfection reagent only were also analyzed. After 72 hours, cell death was quantified using a CellTiter-Blue assay (Promega). AllStars Hs Cell Death Control siRNA resulted in high levels of cell death in both cell types. (Data kindly provided by Dr. Amanda Hummon and Dr. Thomas Reid, National Cancer Institute, Maryland, U.S.A.)
Cell death in primary cells. Primary human NHBE cells (2 x 104) were transfected with 10 nM, 20 nM, or 100 nM AllStars Hs Cell Death Control siRNA or 100 nM nonsilencing siRNA (AllStars Negative Control siRNA) using HiPerFect Transfection Reagent. After 48 hours, cell death was observed by light microscopy.
Easy siRNA transfection optimization. MCF-7 cells (5 x 103) in 96-well plates were transfected with various amounts of AllStars Hs Cell Death Control siRNA or 50 nM nonsilencing siRNA (AllStars Negative Control siRNA) using HiPerFect Transfection Reagent. After 72 hours, cell death was observed by light microscopy. Optimal conditions were 5 nM siRNA and 1.5/2 μl HiPerFect Reagent or 2 nM siRNA and 2 μl HiPerFect Reagent.