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PyroMark Q24 Tests

For analyzing sequence variation or methylation of specific genes using the PyroMark Q24 Instrument
  • Detection and quantification of different mutations in a single run
  • Flexible software for post-run analysis of adjacent mutations
  • Quantification of individual or multiple CpG sites in different genes
  • Estimation of global CpG methylation in defined regions of interest
  • Sequence context provides built-in control of the assay

PyroMark Q24 Tests are a range of assays suitable for highly reliable, quantitative mutation and CpG methylation analysis and are optimized for use with the PyroMark Q24. Tests designated with 'Q24' are optimized for use with the PyroMark Q24, while others such as PyroMark HFE and PyroMark MTHFR can be used with the PyroMark Q96 ID and PyroMark Q96 MD as well. For more tests for these instruments, see PyroMark Q96 Tests.

Cat No./ID: 970012
PyroMark Q24 CpG p16 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene
Cat No./ID: 970022
PyroMark Q24 CpG MLH1 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region –209 to –181 from the transcription start site in the MLH1 gene
Cat No./ID: 970032
PyroMark Q24 CpG MGMT (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene
Cat No./ID: 970042
PyroMark Q24 CpG LINE-1 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements
Cat No./ID: 972432
PyroMark MTHFR (96)
For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
Cat No./ID: 972442
PyroMark HFE (96)
For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
Principle

PyroMark Q24 CpG LINE-1, PyroMark Q24 CpG MGMT, PyroMark Q24 CpG MLH1, and PyroMark Q24 CpG p16 enable highly reliable methylation results. In addition, a built-in control for bisulfite treatment improves accuracy and reproducibility of the results.

Procedure
Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.
Applications

PyroMark Q24 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

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