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artus Enterovirus LC RT-PCR Kit RUO

For quantitative detection of enterovirus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 3.2 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus Enterovirus LC RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation.

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Cat No./ID: 4510003
artus Enterovirus LC RT-PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4510005
artus Enterovirus LC RT-PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Product Details

9
Reliable quantitation of enterovirus load.
Detection of the quantitation standards (Enterovirus LC QS 1–4) on LightCycler 1.1/1.2/1.5 instruments using the artus Enterovirus LC RT-PCR Kit. NTC: no template control.
Performance

To ensure sensitivity, the artus Enterovirus LC RT-PCR Kit has been optimized to detect low numbers of enterovirus RNA. The analytical sensitivity of the kit is 3.2 copies/µl in the PCR.

Performance of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Analytical sensitivity 3.2 copies/µl in the PCR

Principle

The artus Enterovirus LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the enterovirus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus Enterovirus LC RT-PCR Kit provides 4 enterovirus quantitation standards (see figure "Reliable quantitation of enterovirus load". Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical enterovirus RT-PCR is not reduced.

Specifications of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Amplicon 114 bp region of the enterovirus genome

Procedure

The artus Enterovirus LC RT-PCR Kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

Applications
The artus Enterovirus LC RT-PCR Kit enables rapid and sensitive detection of enterovirus RNA from cerebrospinal fluid (CSF) with accurate quantification. The kit is available for use on the Lightcycler 1.1/1.2/1.5 Instruments.

Specifications

Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp Viral RNA Mini Kit
RUO/CE/ASR RUO
Sample type Cerebrospinal fluid (CSF)
Thermal cycler LightCycler
What detected Enterovirus RNA

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