Nucleic acid molecules are separated according to size in gel-filled capillaries.
The indicated amounts of DNA extracted from a lymphoma related cell line (Ramos) were spiked into human leukocyte DNA and the mutated Ramos target was detected together with 2 internal controls. Using the Type-it Mutation Detect PCR Kit, the mutated gene was detected even when only 25 pg of DNA was present. [A] Electrophoresis was performed on a 1.3% agarose gel. [B] Electrophoresis was performed on the QIAxcel Advanced System using the QIAxcel High Resolution Kit. M: 100 bp ladder.
PCR products from a standard PCR were directly analyzed using the QIAxcel DNA Fast Analysis Kit without prior purification.
PCR products were generated using the QIAGEN Multiplex PCR Kit according to the standard protocol. PCR samples (13 µl) were analyzed [A] on a 2% agarose gel and [B] and [C] using the QIAxcel system with the QIAxcel DNA High Resolution gel cartridge and the preinstalled OM500 method. [B] The gel image produced by the QIAxcel system shows much higher resolution than the agarose gel. [C] Each sample lane can be visualized individually in electropherogram form. Lane 7 is shown.
Nucleic acid separation on the QIAxcel Advanced System, using the QIAxcel DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.