RNeasy Mini QIAcube Kit

For automated purification of total RNA from cells and tissues on the QIAcube

Features

  • Fast setup with preloaded rotor adapters
  • Standardized results and streamlined workflow
  • Helps to eliminate loading errors
  • Automated purification from up to 12 samples per run

Useful Resources

RNeasy Mini QIAcube Kit (240)

Cat. No. / ID: 74116

For 240 RNA minipreps: RNeasy Mini Rotor Adapters (preloaded with spin columns and elution tubes); RNase-free reagents and buffers
The RNeasy Mini QIAcube Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The RNeasy Mini QIAcube Kit is a highly convenient format of the RNeasy Mini Kit, dedicated for use on the QIAcube. The RNeasy Mini QIAcube Kit enables purification of consistent yields of high-quality RNA from cells and tissues.

Performance

The RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure " Significant time savings with dedicated QIAcube Kits").
See figures

Principle

The RNeasy Mini QIAcube Kit includes rotor adapters that are preloaded with RNeasy spin columns and elution tubes, increasing ease of use and minimizing user errors. Waste is reduced as the content of the dedicated kit is tailored to purification on the QIAcube. Tubes required for the manual procedure are not included. Using stringent guanidine-isothiocyanate lysis with the purity of silica-membrane purification, the RNeasy Mini QIAcube Kit enables standardized RNA purification from up to 1 x 107 cells or 30 mg tissue.

Procedure

The QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — and up to 12 samples can be processed per run. The QIAcube together with the dedicated RNeasy Mini QIAcube Kit provides fast, easy, and convenient RNA purification.

Applications

The RNeasy Mini QIAcube Kit provides high-quality RNA suitable for downstream applications, such as:

  • Quantitative real-time RT-PCR
  • Array analysis

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPCR, qPCR, real-time RT-PCR, microarray
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinTotal RNA
FormatSpin column, preloaded into rotor adapters
TechnologySilica technology
Sample amount0.5–30 mg
YieldVaries
ProcessingAutomated (QIAcube)
Elution volume30–100 µl
Main sample typeTissue, cells

Resources

Brochures & Guides (1)

FAQ

What is the composition of Buffer RLT?

The exact composition of Buffer RLT is confidential. This buffer is a proprietary component of RNeasy Kits. Buffer RLT contains a high concentration of guanidine isothiocycanate, which supports the binding of RNA to the silica membrane. Buffer RLT can be purchased separately (cat. no. 79216)

Note: note that ß-mercaptoethanol should be added to Buffer RLT before use to effectively inactivate RNAses in the lysate (10 µl ß-Mercaptoethanol per 1 ml Buffer RLT).

FAQ ID -2793
How can I check the integrity of RNA purified using RNeasy Kits?

The integrity and size distribution of total RNA purified with RNeasy Kits can be checked by denaturing-agarose gel electrophoresis, the Agilent 2100 bioanalyzer, or the QIAxcel Advanced System with the QIAxcel RNA QC Kit v2.0.

 

The respective ribosomal species should appear as sharp bands on the stained gel. 28S ribosomal RNA bands should be present with an intensity approximately twice that of the 18S RNA band. If the ribosomal bands are not sharp, but appear as a smear of smaller sized RNAs, it is likely that the RNA sample has suffered major degradation during preparation.

 

Size of ribosomal RNAs from various sources

 

Source rRNA Size (kb) E. coli 16S 1.5   23S 2.9 S. cerevisiae 18S 2.0   26S 3.8 Mouse 18S 1.9   28S 4.7 Human 18S 1.9   28S 5.0

 

 

 

 

 

 

 

 

 

FAQ ID -1024
How much time can be saved when using the RNeasy Mini QIAcube Kit?
Processing your samples with the QIAcube saves 80% hands-on time compared to the manual procedure. Using the RNeasy Mini QIAcube Kit saves another 10% hands-on time compared to processing the classic RNeasy Mini Kit on the QIAcube.
FAQ ID -2335
Are the buffers in the RNeasy QIAcube kit delivered in QIAcube buffer bottles?
No, in order to reduce waste as far as possible the buffers of the RNeasy Mini QIAcube Kit are not delivered in QIAcube buffer bottles but in bigger dimensioned standard QIAGEN buffer bottles. The buffers still have to be transfered into QIAcube buffer bottles by hand.
FAQ ID -2334
Can I buy the RNeasy Mini columns, RNeasy MinElute columns, RNeasy Midi columns or the RNeasy Maxi columns separately?

We do not sell the RNeasy MinElute columns, RNeasy Mini columns, RNeasy Midi columns or the RNeasy Maxi columns separately.

In general, we always provide extra volume of buffers in our kits to account for pipetting errors and such. If you are left with extra buffers after using up all the columns in a kit, please refer to the Material Safety Data Sheet for respective kit to dispose off any unused buffers.

FAQ ID - 3388
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How many samples can be processed per RNeasy Mini QIAcube kit?
The RNeasy Mini QIAcube Kit contains preloaded rotor adapters for 240 samples or 20*12 runs on the QIAcube.
FAQ ID -2332
What dedicated QIAcube Kits are available?
Are QIAshredder spin columns delivered with the RNeasy Mini QIAcube Kit?
No, QIAshredder spin columns are not included in the RNeasy Mini QIAcube Kit but can be ordered separately.
FAQ ID -2333
What is the composition of Buffer RPE?
The exact composition of Buffer RPE is confidential. Buffer RPE is a mild washing buffer, and a proprietary component of RNeasy Kits. Its main function is to remove traces of salts, which are still on the column due to buffers used earlier in the protocol. Ethanol, which is added by the user just before using the kit for the first time, is an important ingredient of Buffer RPE.
FAQ ID -2797
What is the composition of Buffer RW1?

The exact composition of Buffer RW1 is confidential. Buffer RW1 is a proprietary component of RNeasy Kits. Buffer RW1 contains a guanidine salt, as well as ethanol, and is used as a stringent washing buffer that efficiently removes biomolecules such as carbohydrates, proteins, fatty acids etc., that are non-specifically bound to the silica membrane. At the same time, RNA molecules larger than 200 bases remain bound to the column.

Note: Buffer RW1 should not be used for isolation of small RNAs, for example, microRNAs or fragmented RNA from formalin-fixed tissues, as these smaller fragments will be washed away.  Buffer RWT should be used instead.

FAQ ID -2796