RT2 PreAMP cDNA Synthesis Kit
For preamplification of cDNA from small or FFPE samples of RNA for analysis on RT2 Profiler PCR Arrays
The RT2 PreAMP cDNA Synthesis Kit is designed for preamplification of cDNA from nanogram amounts of RNA (1–100 ng) specifically for analysis using RT2 Profiler PCR Arrays.
RT² PreAMP technology utilizes multiplex, tandem PCR to preamplify gene-specific cDNA with minimal bias. The RT2 PreAMP cDNA Synthesis Kit is designed for preamplification of first strand cDNA from 1–100 ng total RNA.
Following standard reverse transcription, the kit works with an array-specific RT2 PreAMP Pathway Primer Mix to preamplify an array-specific set of targets from the cDNA before gene expression analysis using an RT² Profiler PCR Array. Each RNA sample will generate sufficient cDNA for gene expression analysis on up to 4 different RT² Profiler PCR Arrays.
The procedure comprises 2 simple steps:
To complete the PCR array procedure, the preamplified templates are mixed with an instrument-specific, ready-to-use RT² SYBR® Green qPCR Mastermix.
cDNA templates generated using the RT2 PreAMP cDNA Synthesis Kit in conjunction with RT2 PreAMP Pathway Primer Mixes are ready for gene expression profiling using RT2 Profiler PCR Arrays.
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