[A] CoralLoad PCR Buffer contains 2 gel-tracking dyes for improved pipetting visibility during PCR setup, enabling immediate gel loading of PCR products for [B] easy visualization of DNA migration.
The HotStarTaq Plus procedure is fast and easy for maximum convenience.
PCR was performed with HotStarTaq Plus DNA Polymerase, HotStarTaq DNA Polymerase, and Taq DNA Polymerase from QIAGEN, and three hot-start PCR enzymes from the indicated suppliers. Parallel reactions were performed following the suppliers' recommendations, using 50 ng human genomic DNA. A 1.5 kb fragment of the human CFTR gene was amplified in 35 PCR cycles. M: markers.