TransMessenger Transfection Reagent
For efficient mRNA transfection
- Highly efficient transfection
- Reproducible results ensured by stringent quality control
- Efficient transfection of primary neuronal cells
Cat. No. / ID: 301525
Transfection of cells with RNA rather than DNA offers new possibilities for transfection experiments. Transfected RNA sequences are expressed in the absence of transcription, and in a promoter-independent manner. In addition, protein expression usually occurs sooner following transfection of RNA rather than DNA.
RNA transfection with TransMessenger Transfection Reagent can be used for:
|applications||Direct studies of RNA function|
|features||Transfection with RNA. Efficient transfection of neuronal cells.|
|numberofpossibletransfections||80 transfections in 12-well plates / 0,5 ml reagent|
|technology||Lipid-based formulation in conjunction with a RNA-condensing enhancer|
|transfectiontype||Transient transfection, co-transfection|
QIAGEN Transfection Reagents have been used successfully with many different cell types. For your convenience, we have organized data from researchers who have shared their experimental results with us online in our Transfection Cell Database. Simply type your cell line of interest into the 'Search' field on this page, and find transfection results achieved with various QIAGEN Transfection Reagents. Please note that QIAGEN cannot verify data supplied from outside sources.
You can also submit your own transfection data and obtain a gift as appreciation. In addition you can find on our TransFect Protocol Database transfection protocols for specific cell types and plate formats.
Yes, please follow the Supplementary Protocol 'Guidelines for co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent' (TFP21).
In addition, we have also the following Supplementary Protocol for the Attractene Transfection Reagent 'Fast-Forward cotransfection of adherent cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent'.
Unfortunately, we do not have any data for the transfection of siRNA into insect cells. However, we know about customers who have successfully used Effectene Transfection Reagent for the transfection of plasmid DNA into insect cell lines S2 and Sf9 (see FAQ 397). Since Effectene Transfection Reagent and HiPerFect Transfection Reagent for the transfection of eukaryotic cells with siRNA are both lipid based, there is a chance that HiPerFect will work with insect cells. It is especially suitable for transfection of very low siRNA concentrations, reducing the risk of cell toxicity and off-target knockdown effects.
We would appreciate any feedback on your results in case you want to give it a try. Please visit our Transfection Cell Survey page to submit your feedback.
The composition of 1x PBS solution is:
Adjust to a final pH of 7.4.
This solution is not supplied in any QIAGEN Kit, but is used in protocols for various QIAGEN transfection kits.
No, we do not recommend to use RNAiFect for the transfection of plasmid DNA. The RNAiFect Kit does not contain Enhancer which is necessary to form condensed complexes before transfection of DNA into the cell. TransMessenger Transfection Reagent would be the right choice as it allows co-transfection of siRNA and plasmid DNA. Access the protocol via the following link: Co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent
Alternatively, you can use the Attractene Transfection Reagent with the QIAGEN Protocol "Fast -Forward cotransfection of adhernet cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent".