RNA was isolated from 10 mg of various rat tissues stabilized in RNAlater RNA Stabilization Reagent. RNA was purified using QIAzol Lysis Reagent and the QIAcube HT, BioRobot Universal System, or QIAcube. Purified RNA was analyzed by qRT-PCR for pgk1 mRNA using the QuantiFast Probe RT-PCR Kit. CT values indicate similar performance of RNA extracted with the different purification platforms.
RNA was isolated from 10 mg rat tissue (lung, kidney, heart, brain, spleen, and intestine) stabilized in RNAlater RNA Stabilization Reagent. Sample processing was automated on the QIAcube HT, BioRobot Universal System, or the QIAcube using QIAzol Lysis Reagent. A 1 µl aliquot of each purified RNA sample was analyzed on the QIAxcel Advanced using the QIAxcel RNA QC Kit v2.0 and the QX RNA Size Marker 200–6000 nt. The resulting high RNA integrity scores (RIS) indicate recovery of high-quality RNA.