Genomic DNA samples (10 ng each) and non-template control (NTC) samples (containing no template DNA) were amplified using the REPLI-g UltraFast Mini Kit and the DNA yield was determined after various times. An average yield of 7 µg was obtained after amplification for 60 minutes, while 10–13 µg DNA was obtained after 90 minutes. Up to 5 µg DNA was obtained after just 45 minutes, allowing even faster downstream genetic analysis. No DNA was detected in the non-template control reactions.
Amplification of genomic DNA using the REPLI-g UltraFast Mini Kit involves 3 basic steps. First, the sample undergoes gentle alkaline denaturation. Next, the sample is neutralized, and finally incubated with REPLI-g UltraFast DNA Polymerase at 30°C.