While gels can be used to eliminate adapter dimers and contaminating RNAs, there is still a possibility for high prevalence in sequencing reads even after gel excision. On the above left, QIAseq miRNA shows a robust miRNA library with no adapter dimers or contaminating RNA after the basic protocol that includes a bead-based purification. Compared to libraries generated with competitor kits (prior to a required tedious gel excision), the QIAseq-derived miRNA library is much more robust and devoid of adapter dimers and contaminating RNAs.
Collectively, the QIAseq miRNA workflow offers an unrivaled Sample to Insight solution for differential expression analysis and discovery of novel miRNAs using next-generation sequencing.
Starting with total RNA isolated from any sample, the entire QIAseq miRNA Library Kit workflow can be completed in 7 hours. Molecular barcodes are attached during the reverse-transcription reaction. Thus, any library amplification and sequencing biases can be accounted for.
The QIAseq miRNA Library Kit has been designed to enhance yields from biofluids such as serum. This figure shows robust detection of miRNA from serum samples.
This figure shows mapped reads compared to adapter dimers in serum samples. QIAseq miRNA still shows superior mapping of miRNAs even with limited samples.