Investigator HDplex and Triplex Kits

For multiplex amplification of supplementary autosomal STR loci for reliable differentiation from related individuals, or forensic stain pre-selection

Features

  • Supplementary autosomal STR markers with high discriminatory power for special questions in human identification or kinship analysis
  • Cost-saving screening assays for pre-selection of forensic stains
  • Highly suited for reference or casework samples
Investigator HDplex Kit (100)

Cat. No. / ID: 381215

Primer Mix, Reaction Mix, Multi Taq2 Polymerase, Control DNA, DNA Size Standard 550 (BTO), Allelic Ladder HDplex, and Nuclease-Free Water
$1,900.00
KitAccessories
Investigator STR Kit
Matrix Standard
Product type
HDplex
Triplex AFS
Triplex DSF
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Investigator HDplex and Triplex Kits are intended for molecular biology applications in forensic, human identity, and paternity testing. These products are not intended for diagnosis, prevention or treatment of a disease.

Product Details

The Investigator HDplex Kit amplifies 12 autosomal STR markers, most of which are not featured in commonly used marker standards. This enables the reliable differentiation of forensic samples derived from related individuals and gives heightened discriminatory power in these demanding cases. The genetic analysis is calibrated with BT5 Matrix.

Investigator Triplex Kits are for forensic stain pre-selection, which serves as a starting point for genotyping strategies. They enable simultaneous amplification of STR loci D3S1358, SE33 (ACTBP2) and FGA (FIBRA) (Investigator Triplex DSF) or FGA (FIBRA), SE33 (ACTBP2) and Amelogenin (Investigator AFS QS Kit) from forensic or reference samples. The genetic analysis is calibrated with the DS-30 matrix.

Investigator STR Kits meet ISO 18385 requirements.

Performance

Investigator HDplex and Triplex Kits have been validated using the GeneAmp 9700 thermal cycler and the Applied Biosystems 3130 Genetic Analyzer.

The recommended amount of DNA under standard conditions is 0.35 ng. Internal validations using the Investigator HDplex Kit demonstrated robust and balanced results with 0.2–1.0 ng DNA, and reliable results with <0.1 ng DNA. Internal validations using Investigator Triplex Kits demonstrated robust and balanced results with 0.1–1.0 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator HDplex Kit enables the simultaneous amplification of 12 STR markers (D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D21S2055, SE33) and the gender-specific Amelogenin. These markers include some highly polymorphic STR markers that are not included in commonly used standards enabling higher discrimination in difficult cases.

The Investigator Triplex AFS QS Kit enables the simultaneous amplification of the two polymorphic STR loci FGA (FIBRA) and SE33 (ACTBP2), and the gender-specific Amelogenin. The kit also includes the Quality Sensor (QS). This special feature is an internal PCR control that provides information on the presence of PCR inhibitors and the efficiency of the PCR.

The Investigator Triplex DSF Kit enables the simultaneous amplification of the 3 polymorphic STR loci D3S1358, SE33 (ACTBP2) and FGA (FIBRA).

Procedure

With the Investigator HDplex Kit, the 12 polymorphic STR markers and Amelogenin are simultaneously amplified and labeled with the dyes listed in the table "Investigator HDplex Kit dyes and corresponding markers".

Using the Investigator Triplex AFS QS Kit, FGA (FIBRA), SE33 (ACTBP2) and Amelogenin are simultaneously amplified in a single PCR and labeled with 6-FAM.

With the Investigator Triplex DSF Kit, the 3 polymorphic STR loci are amplified simultaneously and labeled with the fluorescent dyes as listed in the table "Investigator Triplex DSF dyes and corresponding markers".

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystem’s GeneMapper ID-X Software. See the table "Technical specifications" for more information.

Investigator HDplex Kit dyes and corresponding markers

Dye Markers            
6-FAM Amelogenin D7S1517 D3S1744 D12S391  D2S1360 D6S474 D4S2366
BTG D8S1132 D5S2500 D18S51 D21S2055      
BTY D10S2325 SE33          

Investigator Triplex DSF Kit dyes and corresponding markers

Dye Markers  
6-FAM D3S1358 SE33 (ACTBP2)
HEX FGA  

Applications

The Investigator HDplex Kit enables reliable generation of DNA profiles from blood, buccal swabs and forensic stains. Thanks to the STR markers used, this kit is highly suited for difficult forensic cases involving related individuals, difficult paternity cases, population genetic studies and other human identity testing applications involving related individuals.

Investigator Triplex Kits are preferentially used for forensic prescreening strategies or for low-copy and degraded DNA samples that do not give distinct results with complex multiplexes. They enable rapid and reliable DNA genotyping of blood, buccal swabs and forensic stains.

Technical specifications

Features Investigator HDplex Kit Investigator Triplex AFS QS Kit Investigator Triplex DSF Kit
Volume per amplification reaction 25 μl 25 μl 25 μl
Matrix BT5 DS-30 DS-30
Fluorescence labels 6-FAM, BTG, BTY, BTO 6-FAM, ROX 6-FAM, HEX, ROX
Genetic analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers ABI PRISM 3100/3100-Avant, Applied Biosystems 3130/3130xl, or Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis Applied Biosystems GeneMapper or GeneMapper ID-X Applied Biosystems GeneMapper ID or GeneMapper ID-X Applied Biosystems GeneMapper ID or GeneMapper ID-X

Resources

Technical Information (3)

Enjoy success at first try with high performance QIAGEN Multiplex STR amplification and instrumentation

Quality initiatives for human identity testing and forensics 
Template Files (8)
For further information on which version of the v10 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
For further information on which version of the v11 template files is suitable for your needs, please refer to the Template File Info Note.
Brochures & Guides (2)
Advanced sample collection, automation and STR solutions for kinship testing

FAQ

3197 -Where can I find the Population Data for the Investigator HDplex kit?
The Population data excel file can be found on the “Resources” tab on the product webpage.
What fluorescent labels are 6-FAM, BTG, BTY, BTR, and BTO in Matrix Standard BT5 single or multi.cap?

The BT5 matrix standard is designed for use with our Investigator STR and Dipplex Assays. We do not disclose the containing dyes.

FAQ ID - 3364