Investigator 24plex GO! Kit

The Investigator 24plex GO! Kit utilizes fast-cycling technology to provide reliable results in approximately 45 minutes directly from the sample without prior DNA extraction.

The novel Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology that has been further optimized for direct amplification. Assay performance tested in the presence of increasing concentrations of inhibitors shows superior inhibitor resistance. This enables reliable, high-quality results from direct amplification of typical reference samples such as blood.

The Investigator 24plex GO! Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.

The Investigator 24plex GO! Kit uses QIAGEN's fast-cycling PCR technology for direct, simultaneous amplification of the CODIS and ESS markers, plus SE33, DYS391, D2S1338, D19S433 and Amelogenin. Carefully designed primers minimize the overlap of alleles of different makers, thereby minimizing the likelihood of data misinterpretation.
The innovative Quality Sensors provide additional information on sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:

• If amplification was successful
• If DNA was present in the sample
• If the sample was degraded
• If inhibitors in the sample prevented successful PCR

This additional information will greatly enhance your data analysis and will help you to make crucial decisions with respect to further sample processing. Ultimately, the Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.

The optimized design of the two gender markers, Amelogenin and DYS391, enables the Investigator 24plex GO! Kit to have both at a small fragment size, making the gender typing extremely robust. Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.

The STR markers and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator 24plex GO! Kit dyes and corresponding markers".

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Investigator 24plex GO! Kit technical specifications" for more information.

Investigator 24plex GO! Kit dyes and corresponding markers

Dye	Marker
6-FAM	Amelogenin	TH01	D3S1358	vWA	D21S11
BTG	TPOX	DYS391	D1S1656	D12S391	SE33
BTY	D10S1248	D22S1045	D19S433	D8S1179	D2S1338
BTR2	D2S441	D18S51	FGA
BTP	QS1	D16S539	CSF1PO	D13S317	D5S818	D7S820	QS2

 

Investigator 24plex GO! Kit technical specifications

Category	Details
Volume per amplification reaction	20 μl
Matrix	BT6
Fluorescence labels	6-FAM, BTG, BTY, BTR2, BTO, BTP
Genetic analyzers	Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis	Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software

The Investigator 24plex GO! Kit is for direct and reliable generation of DNA profiles from blood on FTA or other paper, buccal cells on FTA or other paper, or buccal swabs. It is highly suited for:

• Criminal database samples
• Paternity testing
• Population genetics studies
• Other human identification and forensics applications