Want to try the QuantiNova SYBR Green PCR Kit for the first time? Request a quote for a trial kit.
Want to try the QuantiNova Probe PCR Kit for the first time? Request a quote for a trial kit.
Want to try QuantiNova Multiplex PCR Kits for the first time? Request a quote for a trial kit
Built-in visual indicator for accurate reaction setup
The master mix supplied with QuantiNova PCR Kits contains an inert blue dye that does not interfere with the real-time PCR, but increases visibility in the tube or well. The QuantiNova Yellow Template Dilution Buffer contains an inert yellow dye. When template nucleic acid is diluted in QuantiNova Yellow Template Dilution Buffer and added to the master mix, the color of the solution changes from blue to green (see figure “ Accurate reaction setup indicated by the built-in pipetting control”), providing a visual indication that each reaction was set up correctly.
QuantiNova SYBR Green PCR Kit
The high sensitivity of the QuantiNova SYBR Green PCR Kit results in accurate and robust detection of even a single target copy (see figure “ Robust and sensitive detection of single-copy targets”). This extreme sensitivity is achieved even under ultrafast cycling conditions.
The QuantiNova SYBR Green PCR Kit can be used with a wide range of template amounts. The performance of this SYBR real-time PCR kit has been tested for a dynamic range spanning 8 orders of magnitude (from 100 ng to 10 fg cDNA) and regardless of instrument used, quantification was accurate and robust (see figure “ Accurate quantification over a wide dynamic range”).
The stringent specificity and exceptional sensitivity of the QuantiNova chemistry produces optimal results on the QIAGEN Rotor-Gene Q, but can be used on any real-time PCR cycler, regardless of format, fast-cycling capacity, and need for a passive reference dye. ROX provided with the QuantiNova SYBR Green PCR Kit is simply added to the master mix if required. Amplification and quantification results using the Rotor-Gene Q, Agilent Technologies Mx3005P, Applied Biosystems 7900 HT Fast, ViiA 7, StepOnePlus, and 7500 Fast, Roche LightCycler 480, and Bio-Rad CFX96 are invariably robust and sensitive.
The QuantiNova SYBR Green PCR Kit delivers superior results compared to other SYBR Green PCR kits. Regardless of cycling conditions or instrument, the QuantiNova chemistry demonstrably generates lower CT values, higher reproducibility, and higher reaction efficiency.
The QuantiNova Yellow Template Dilute Buffer provides peace of mind during reaction setup but is not required to obtain superior results with the QuantiNova SYBR Green PCR Kit. The QuantiNova chemistry is designed to be flexible, so it can be incorporated into any established workflow. Thus, performance is optimal with your amplification target of interest, and your instrument and dilution buffer of choice.
QuantiNova Probe PCR Kit
The real-time PCR mix of this probe real-time PCR kit can be stored at 30°C for up to 100 hours without impairing the performance of subsequent reactions. The outstanding stability, even after extended storage at room temperature without the use of any cooling agent, makes the QuantiNova Probe PCR Kit ideal for high-throughput reaction setup and plate-stack handling (see table "Effect of storage on reaction stability".
Effect of storage on reaction stability
|Mean CT values
||QuantiNova Probe PCR Kit
||Probe PCR kit from supplier L
|Amount in ng
|| 0 h
|| 100 h
|| 0 h
|| 100 h
|No template control
Comparison of CT values before and after 100 hours storage at 30°C. Real-time PCRs including master mix, template cDNA, primers, and probes for TNF were incubated at 30°C for 100 hours and run in the real-time PCR together with freshly prepared reactions. Triplicate reactions of 3 different template amounts were tested. In contrast to the probe PCR kit from supplier L, the CT values provided by the QuantiNova Probe PCR Kit did not vary after extended storage of the samples at 30°C.
The high sensitivity of the QuantiNova Probe PCR Kit results in accurate and robust detection of even a single target copy (see figure “ Sensitive and robust detection of a single target copy”). The special master mix supplied with these kits enables accurate quantification of 2 targets having widely differing abundance in a single tube. This saves time, money, and reduces the amount of sample material needed. Moreover, the duplex PCR data obtained is comparable with that obtained from a singleplex PCR.
The unique combination of QIAGEN's proprietary and well-proven buffer technology along with the new QuantiNova DNA Polymerase, QuantiNova Antibody, and QuantiNova Guard, ensures real-time PCR success at the first attempt, without the need for costly and time-consuming optimization, even with challenging real-time PCR assays (see figure “ Superior results with challenging assays”).
The QuantiNova Probe PCR Kit can be used on any real-time cycler. ROX is provided in a separate tube and can be added if using a cycler that uses ROX as a passive reference dye. The kit delivers highly consistent results among different cyclers in terms of sensitivity, reproducibility, and efficiency. The consistency of the results is maintained, despite varying fast-cycling capacities of the different cyclers that result in different cycling protocols.
QuantiNova Multiplex PCR Kits
The special master mix supplied with QuantiNova Multiplex PCR Kits allows rapid setup of multiplex reactions and delivers successful results at the first attempt, providing multiplex PCR data that are comparable with singleplex PCR data. The highly concentrated 4x master mix accommodates up to 800 ng template input ensuring outstanding sensitivity, even in up to 5plex reactions. The kit can clearly distinguish between small differences in the amount of template and provides accurate quantification of targets of widely differing abundance.
A novel, antibody-mediated, hot-start mechanism ensures outstanding specificity and allows reaction setup at room temperature, ideally suited for automated procedures. The specially developed fast PCR buffer contains the additive Q-Bond, which significantly reduces annealing and extension times, allowing multiplex qPCR in less than one hour. The visual pipetting control helps to prevent human errors and increases process safety, particularly if combined with the Internal Control RNA provided in the QuantiNova Reverse Transcription Kit for quantitative 2-step RT-PCR.
The QuantiNova Multiplex PCR Kit increases workflow efficiency by generating more insight from limited sample material by using ultrafast and in-process controlled multiplex qPCR.