Sharper dissociation curves with RT2 SYBR<sup>®</sup> Green FAST Mastermix.
Sharper dissociation curves with RT2 SYBR® Green FAST Mastermix.
High amplification efficiency over a wide dynamic range.
High amplification efficiency over a wide dynamic range.
Tighter control of polymerase activity yields greater specificity.
Tighter control of polymerase activity yields greater specificity.
Sharper dissociation curves with RT2 SYBR<sup>®</sup> Green FAST Mastermix.

A positive PCR control was amplified on the ABI 7500 qRT-PCR instrument using [A] regular cycling and RT² SYBR Green Mastermix or [B] fast cycling with RT² SYBR Green FAST Mastermix. The dissociation curve obtained with RT² SYBR Green FAST Mastermix has lower background and a sharper shape.
High amplification efficiency over a wide dynamic range. Using duplicate 10-fold serial dilutions of purified template and the RT2 qPCR Primer Assay for the Human Nicotinic Acetylcholine Receptor Alpha 5 (CHRNA5), RT2 SYBR® Green Mastermixes demonstrate a 6-log linear dynamic range.
Tighter control of polymerase activity yields greater specificity. Greater specificity was achieved using RT2 SYBR® Green Mastermixes [A] compared to master mixes from an alternative supplier [B].