Raw Cq values from two separate RT reactions (RT1 and RT2) on total RNA purified from 65 µl serum are shown. A total of 730 miRNAs were profiled. Only miRNAs with Cq values below 35 have been included (133 data points).
Different RT reactions using 40 ng heart and liver total RNA were profiled on the miRCURY LNA miRNome Human PCR Panel I and II on different days. The correlation between raw Cq values from all miRNAs with signals below 35 Cq values is shown (total of 297 data points).
Aliquots of the same heart and liver total RNA were expression profiled using miRCURY LNA miRNA PCR Panels in two different locations (on separate days by different operators) using different Roche LightCycler 480 Real-Time PCR instruments. The correlation between average Cq values (triplicate RT reactions) from all miRNAs with signals below 35 Cq values (total of 297 datapoints) is shown.