Analysis of PCR products before (b) and after (a) purification with the QIAquick PCR Purification Kit is shown. Samples were analyzed on a 1% TAE agarose gel. M: markers.
pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
Manifold with luer connectors.
GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
QIAvac 24 plus.
The QIAquick and MinElute systems use a simple bind-wash-elute procedure with spin columns or a vacuum manifold.