Human blood was collected from 12 donors and treated with EDTA. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. The mean values of 3 independent runs are indicated.
PCR products (2 µg in a volume of 100 µl) were purified using the QIAquick PCR Purification Kit with the manual procedure or automated on the QIAcube. Percentage recovery was determined spectrophotometrically. Recovery of PCR products using the QIAcube was comparable to the manual procedure.
pCMVβ plasmid DNA was purified from 3 ml overnight cultures of E. coli (DH5α) grown in LB medium using the QIAprep Spin Miniprep Kit automated on the QIAcube or by using the manual procedure. DNA yields were determined spectrophotometrically. Automated DNA purification on the QIAcube gave comparable yields to the manual procedure.
RNA was purified from 1 x 106 Jurkat cells using the RNeasy Mini Kit on the QIAcube. RNA yields were consistent, demonstrating the efficiency and reproducibility of the automated procedure.
Using the QIAcube. Fully automated purification of nucleic acids or proteins using QIAGEN spin kits, such as QIAprep Mini Kits, on the QIAcube eliminates the need for tedious manual steps. Sample preparation on the QIAcube follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute). No change in purification chemistry is required.
Human blood was collected from 3 donors and treated with one of 3 anticoagulants: EDTA, citrate, or heparin. Genomic DNA was purified from 200 µl blood using the QIAamp DNA Blood Mini Kit on the QIAcube. DNA was eluted in 200 µl. Yields of genomic DNA were determined by measuring the absorbance at 260 nm. A 1.2 kb fragment of the single-copy Hugl gene was amplified using 1 µl, 5 µl, or 10 µl purified DNA in a final reaction volume of 25 µl. Amplification reactions were performed using HotStarTaq DNA Polymerase. A 5 µl aliquot of each PCR was run on a 1.5% agarose gel. M: 100 bp ladder; +: positive control; –: negative control.
6xHis-tagged green fluorescent protein (GFP) was purified in duplicate from 5 ml E. coli cultures under native conditions using the Ni-NTA Spin Kit with the manual procedure or automated on the QIAcube. Proteins were visualized by Coomassie staining after SDS-PAGE. A 10 µl aliquot of eluate was loaded. M: marker.
RNA was purified using the RNeasy Mini Kit with the manual procedure or automated on the QIAcube. RNA was purified from 1 x 106 cells (RNeasy standard, RNeasy DNase digestion); 5 x 106 cells (RNeasy QIAshredder); 25 µg total RNA (RNeasy cleanup). Purified RNA was analyzed on the Agilent 2100 bioanalyzer. The high RNA Integrity Number (RIN) indicates the high quality of the RNA.
pUC19 Plasmid DNA was purified from a 1.5 ml overnight culture of E. coli grown in LB medium using the QIAprep Spin Miniprep Kit on the QIAcube and sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit. Sequencing reactions were purified using the DyeEx 96 Kit and analyzed on an ABI PRISM 3700 DNA Analyzer.
QIAcube was given the design industry’s “Red Dot Award” recognizing the platform’s innovation, functionality, quality and ergonomics.
The QIAcube - QIAGEN’s revolutionary new platform for sample preparation - receives the Association for Laboratory Automation’s (ALA) New Product Award (NPA).