PCR reactions were run using the AllTaq Master Mix Kit with different targets, varying template amount and annealing temperature (from 50°C to 60°C). Analysis was performed on the QIAxcel Advanced. All reactions resulted in successful and specific amplification of all targets.
The AllTaq Master Mix Kit was used to amplify two DDX31 amplicons; 7644 bp and 8932 bp. Analysis was performed by agarose gel electrophoresis. Arrows indicate specific product.
The gDNA template was diluted to theoretical one copy per reaction for amplification of CFTR (228 bp amplicon) using the AllTaq Master Mix Kit. A total of 68 parallel PCR reactions were run and then analyzed using the QIAxcel Advanced. 42 positive reactions were achieved experimentally, which is highly consistent with the 43 positive reactions predicted theoretically, using Poisson’s equation. Positive reactions are indicated with a "+", and negative reactions are indicated with a "–". This comparison demonstrates reliable and highly sensitive single copy detection with the AllTaq Master Mix Kit.
The AllTaq PCR Kit allows fast and easy PCR setup – just mix all components together in one tube and start your thermal-cycler program. The reaction mixture contains all of the reagents required for PCR. As a visual pipetting control, you can add the optional orange Master Mix Tracer and blue Template Tracer, verifying correct reaction setup, and allowing tracking during gel electrophoresis.