miScript miRNome for biomarker discovery
Cat No./ID: 4E76E090-48F7-4445-80A4-64B8C1697385
miRNome miScript miRNA PCR ArrayGo to GeneGlobe
miRNome panels of miRNA assays in 96-well, 384-well, or Rotor-Disc formats
Discover biomarkers in serum or plasma samples
The range of miScript miRNA PCR Arrays is continually expanding to enable new discoveries about the roles of miRNAs in biological processes. Qiagen has taken biomarker discovery beyond the individual pathways to entire miRNA profiling with the miRNome. The content of miScript miRNA PCR Arrays is selected using our proprietary methodology, which ensures that each array is up-to-date and biologically relevant. One of the most exciting areas of current miRNA research involves the assessment of miRNAs present in serum or plasma samples. The discovery of relatively stable, extracellular miRNAs in serum and plasma has generated huge interest in the use of changes in these miRNA levels as potential non-invasive biomarkers for a variety of diseases. As a result, the Serum & Plasma miScript miRNA PCR Array has been developed. This array enables rapid profiling of the 84 most relevant miRNAs associated with serum and plasma (see figure "Analysis of expression of circulating miRNAs in serum or plasma").
Profile the human miRNome: start with a little, get a lot!
The Human miRNome miScript miRNA PCR Array profiles the expression up to 2500 of the most abundantly expressed and best characterized miRNA sequences in the human miRNA genome (miRNome) as annotated in miRBase Release 21 (www.mirbase.org). With less than 1 µg RNA, the entire human miRNome can be rapidly profiled (see figure "Whole miRNome profiling"). Although they are well characterized, each of these miRNA sequences can regulate one to several messenger RNA transcripts, and conversely one mRNA can be regulated by one to several miRNA sequences. Therefore, the complex role that any given known miRNA sequence plays has yet to be completely defined. Each assay in a miScript miRNA PCR Array has been bench verified to ensure sensitive and specific detection of mature miRNA by real-time PCR.
The miScript PCR System enables sensitive, specific miRNA quantification and profiling using SYBR Green real-time PCR. The miScript PCR System covers all the steps involved in conversion of RNA to cDNA and subsequent real-time PCR detection of miRNAs.
Mature miRNA profiling with the miScript PCR System uses the following components:
miScript miRNA PCR Array plate layout
miScript miRNA PCR Arrays are available in 96-well, 384-well and Rotor-Disc 100 formats (see figures "Pathway-Focused or miRNome miScript miRNA PCR Array layout for plate formats A, C, D, F, M", "Pathway-Focused miScript miRNA PCR Array layout for plate formats E, G", "miRNome miScript miRNA PCR Array and miScript miRNA HC PCR Array layout for plate formats E, G" and "miScript miRNA PCR Array layout for Rotor-Disc format R"). Each array contains several control assays. The purpose of these assays is described in the table below.
Controls in miScript miRNA PCR Arrays
C. elegans miR-39 miScript Primer
Assay Alternative data normalization using
exogenously spiked Syn-cel-miR-39
miScript miRNA Mimic 6 snoRNA/snRNA miScript Primer
Assays (miScript PCR Controls) Data normalization using the ΔΔCT
method of relative quantification miRNA reverse transcription control
(miRTC) miScript Primer Assay Assessment of reverse transcription
performance Positive PCR control (PPC) Assessment of PCR performance
For more information on these controls, consult the miScript miRNA PCR Array Handbook.
miScript miRNA QC PCR Array plate layout
miScript miRNA QC PCR Arrays enable assessment of the quality of multiple cDNA samples using real-time PCR. miScript miRNA QC PCR Arrays are available in 96-well, 384-well, and Rotor-Disc 100 formats (see figures miScript miRNA QC PCR Array layout for plate formats A, C, D, F, miScript miRNA QC PCR Array layout for plate formats E and G, and "miScript miRNA QC PCR Array layout for Rotor-Disc format R"). Each miScript miRNA QC PCR Array includes replicates of C. elegans miR-39 miScript Primer Assay, 3 mature miRNA miScript Primer Assays (miR-16, miR-21, miR-191), 3 snoRNA miScript Primer Assays (SNORD61, SNORD95, SNORD96A), miRTC miScript Primer Assay and a positive PCR control. For more information, consult the miScript miRNA PCR Array Handbook. The 96-well plate and Rotor-Disc 100 formats allow quality control of up to 8 cDNA samples. The 384-well format allows quality control of up to 32 cDNA samples.
miScript miRNA PCR Array Data Analysis Tool
Free, easy-to-use data analysis software is available online for miScript miRNA PCR Arrays. Each miScript miRNA PCR Array comes with a dedicated data analysis tool. Once raw CT values are entered, the analysis tool automatically interprets the PCR array controls, performs relative quantification using the ∆∆CT method and presents the results in a variety of visual formats, allowing you to rapidly interpret your data.
miScript miRNA PCR Arrays are used as part of the miScript PCR System for: