DNeasy mericon 96 QIAcube HT Kit

For automated high-throughput isolation of DNA from raw or processed food samples

Features

  • Purification of DNA from various types of food samples
  • No carryover of PCR inhibitors from complex food matrices
  • Accelerated CTAB procedure ensures efficient DNA extraction
  • Simple and reliable automated processing for cost and time savings
DNeasy mericon 96 QIAcube HT Kit (5)

Cat. No. / ID: 69571

For 480 preps: DNeasy 96 plates, QIAGEN Proteinase K, buffers
NOK 10,505.00
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DNeasy mericon 96 QIAcube HT Kit
QIAcube HT Plasticware
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The DNeasy mericon Food Kit is intended for molecular biology applications in food, animal feed, and pharmaceutical product testing. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details


The DNeasy mericon 96 QIAcube HT Kit enables simple, automated purification of DNA from a variety of raw and processed foods on the QIAcube HT system. Using proven DNeasy silica-membrane technology in a convenient 96-well format, contaminants and inhibitors from complex food matrices are removed to yield high-quality DNA ready for downstream analysis. The kit forms part of the comprehensive QIAGEN food testing portfolio, which also features assays for pathogen and GMO DNA detection and ingredient authentication.

Performance

The DNeasy mericon 96 QIAcube HT Kit is designed for rapid, automated purification of DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. DNeasy mericon purification kits provide a universally applicable extraction method that gives reliable results even when using strongly inhibitory, highly processed, fatty, acidic, high-, or low-DNA content foods (see figure  High yields of DNA and minimal inhibitor carryover). The QIAcube HT platform, together with the dedicated DNeasy mericon 96 QIAcube HT Kit, enables automated mid- to high-throughput DNA purification from food sample types. Now it is possible to prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure (see figure  Purification of DNA from food samples).
See figures

Principle

The DNeasy mericon 96 QIAcube HT Kit enables automated purification of nucleic acids from up to 300 mg food sample material using the QIAcube HT instrument. The procedure yields high-quality DNA that performs well in PCR and other downstream applications.

The DNeasy mericon 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
 
Kit specifications
Specification Description
Number of samples 24–96 samples (to be processed in increments of 8)
Sample input volume Up to 300 mg food material
Elution volume 200 μl
Duration 96 samples in approximately 73 minutes
24 samples in approximately 42 minutes

Procedure

The DNeasy mericon 96 QIAcube HT Kit uses a modified cetyltrimethylammonium bromide (CTAB) extraction procedure for rapid automated purification of DNA from food matrices on the QIAcube HT instrument (see figure  Efficient and rapid automated DNA extraction). Homogenized food samples undergo a manual pretreatment step for efficient lysis using Food Lysis Buffer in combination with proteinase K. Chloroform extraction removes any CTAB-protein, CTAB-debris, or CTAB-polysaccharide complexes. The aqueous phase containing the DNA is transferred to a 96-well S-Block, and all subsequent  purification steps are automated on the QIAcube HT instrument. 

QIAcube HT adds Buffer PB to the aqueous phase and transfers the mixture to the DNeasy 96 plate. As vacuum is applied, nucleic acids are adsorbed onto the silica membrane while contaminants pass through. Washing removes any remaining contaminants and inhibitors, and the DNA is eluted in Buffer EB. The resulting purified DNA is ready for use in a downstream mericon real-time PCR assay.
See figures

Applications

The DNeasy mericon 96 QIAcube HT Kit yields high-quality DNA from a variety of raw and processed food matrices, while minimizing the carryover of PCR inhibitors inherent to complex food samples. Purified DNA is suitable for a range of downstream applications:
  • Food safety and quality testing
  • Ingredient authentication
  • Detection of genetically modified organisms
  • Detection of potential allergens

Supporting data and figures

Resources

Protocol Files (5)

Interim version of QIAamp 96 PowerFecal protocol to be used with QIAamp 96 PowerFecal QIAcube HT Kit.

Interim version of QIAamp 96 Virus protocol to be used with QIAamp 96 Virus QIAcube HT Kit.

Interim version of QIAamp 96 DNA protocol to be used with QIAamp 96 DNA QIAcube HT Kit.

Q Protocol notes for purification of total DNA from food samples on the QIAcube HT using the DNeasy mericon 96 QIAcube HT Kit; use with the DNeasy mericon 96 QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.
Quick-Start Protocols (2)
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software
Kit Handbooks (1)
Handbook for purification of total DNA from food samples on the QIAcube HT using the DNeasy mericon 96 QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.
Brochures & Guides (1)
For fast and reliable automated 96-well nucleic acid purification
Instrument User Manuals (1)
Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699