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Investigator 26plex QS Kit

For multiplex amplification of all Chinese National database loci and CODIS-required markers, with an innovative Quality Sensor
  • Chinese, CODIS and European standard markers in one reaction
  • Integrated Quality Sensor to support decision making
  • Optimized FRM 3.0 chemistry for enhanced robustness and stability
  • Very high discrimination power provided by the 25 loci
  • Reduced allelic overlap and unambiguous results

 

The Investigator 26plex QS Kit is for human identification, allowing multiplex amplification of all Chinese National database loci, CODIS-required markers, and Amelogenin. Due to the choice of markers that also includes Penta E and Penta D, the kit offers best-in-class discrimination power. Furthermore, the kit consists of the innovative Quality Sensor, which means that the Investigator 26plex QS Kit generates additional, valuable data for your quality control and performance checks. The kit uses QIAGEN's latest fast-cycling technology FRM 3.0, which not just enables PCR in just 60 minutes, but also provides enhanced robustness towards DNA overloading and stability. Highly sensitive, yielding rapid and reliable results from trace DNA, the Investigator 26plex QS Kit is suitable for all forensic applications and paternity testing. Once you've tried it, you'll always insist on Quality Sensor!

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Cat No./ID: 382615
Investigator 26plex QS Kit (100)
kr19,320.00
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Primer Mix, Fast Reaction Mix 3.0 including Taq DNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water
Cat No./ID: 382617
Investigator 26plex QS Kit (400)
kr69,835.00
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Primer Mix, Fast Reaction Mix 3.0 including Taq DNA Polymerase, Control DNA, allelic ladder 26plex, and nuclease-free water
Cat No./ID: 386035
DNA Size Standard 24plex (BTO) (100)
kr980.00
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DNA Size Standard 24plex (BTO) for 100 reactions
Cat No./ID: 386045
DNA Size Standard 450 (BTO) (100)
kr980.00
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DNA Size Standard 450 (BTO) for 100 reactions; 13 fragments, higher fluorescence and adjusted for high concentration of DNA input
The Investigator 26plex QS Kit is intended for molecular biology applications in forensic, human identity, and paternity testing. This product is not intended for diagnosis, prevention, or treatment of a disease.

 


Product Details

Performance

The Investigator 26plex QS Kit utilizes our latest FRM 3.0 technology and provides reliable results in approximately 60 minutes, which is significantly faster than kits from other suppliers. The new chemistry makes the kit more robust towards DNA overloading whenever no DNA quantification is done and provides a very good stability of the Mastermix when stored at 4°C.

Furthermore, it is based on proven QIAGEN multiplex PCR technology that has been further optimized for ease of handling and increased sensitivity. Assay performance tests in the presence of increasing concentrations of inhibitors showed both high sensitivity and superior inhibitor resistance. This means reliable, high-quality results.

The Investigator 26plex QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer. The recommended amount of DNA under standard conditions is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2 to 6 ng DNA, and reliable results with <0.1 ng DNA.

Principle

The Investigator 26plex QS Kit uses QIAGEN's fast-cycling PCR technology for the rapid, simultaneous amplification of the Chinese, CODIS and European standard markers, as well as Amelogenin. Carefully designed primers minimize the overlap of alleles of different makers, thereby minimizing the likelihood of misinterpretation of data.

The innovative Quality Sensors provide additional information on the sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine:

  • If the PCR amplification worked
  • If there was any DNA in the sample
  • If the sample was degraded
  • If inhibitors in the sample prevented successful PCR

This additional information will greatly enhance how you analyze your data and aid you in making crucial decisions with respect to further sample processing. Ultimately, the Quality Sensors will help you reduce unnecessary re-runs, saving you time and money.

The optimized design of the two gender markers, Amelogenin and DYS391, enables the Investigator 26plex QS Kit to have both at a small fragment size, making the gender typing extremely robust. Even for populations with Amelogenin Null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.

Procedure

The STR markers, Quality Sensor and the gender-specific Amelogenin are amplified simultaneously in a single PCR and labeled with one of the dyes listed in Table 1.

The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the kit technical specifications in Table 2 for more information.

Table 1. Investigator 26plex QS Kit dyes and corresponding markers
Dye Marker            
6-FAM Amelogenin TH01 D3S1358 Penta D D6S1043  D21S11  
BTG TPOX DYS391 D1S1656 D12S391 Penta E    
BTY D10S1248 D22S1045 D19S433 D8S1179 D2S1338    
BTR2 D2S441 D18S51 vWA FGA      
BTP QS1 D16S539 CSF1PO D13S317 D5S818 D7S820 QS2

 

Table 2. Investigator 26plex QS Kit technical specifications
Category Details
Volume per amplification reaction 25 μl
Matrix BT6
Fluorescence labels 6-FAM, BTG, BTY, BTR2, BTO, BTP
Genetic analyzers Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software
Applications

The Investigator 26plex QS Kit is for rapid and reliable generation of DNA profiles from blood, buccal swabs and forensic stains. It is highly suited for:

  • Criminal database and forensic casework samples
  • Paternity testing
  • Population genetics studies
  • Other human identification and forensics applications

The Investigator 26plex QS Kit provides the full flexibility for any kind of application and use because the DNA Size Standard is purchased separately: DNA Size Standard 450 (BTO), cat. no. 386045, with 13 fragments of higher fluorescence and adjusted for high concentration of DNA input/no quant; or DNA Size Standard 24plex (BTO), cat. no. 386035, with 26 fragments, standard fluorescence, dedicated for standard applications (casework or reference samples) in human ID and forensics.

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