For rapid and economical automated purification of total DNA from plant tissue
Complete removal of contamination and inhibitors
Pure DNA, ready for use in genotyping applications
Automated procedure saves time and effort
No organic extraction or alcohol precipitation
The BioSprint 96 DNA Plant Kit provides automated purification of total DNA from plant tissue on the BioSprint 96 using proven MagAttract magnetic particle technology. The kit contains all reagents and plasticware necessary for automated DNA purification.
For 1536 preps: Large 96-Rod Covers, 96-Well Microplates MP, S Blocks, MagAttract Suspension G, buffers and reagents
Reproducible purification of high-quality DNA.
Genomic DNA was purified from 50 mg samples of fresh leaf tissue from [A] arabidopsis, [B] maize, and [C] rose. Purification was performed using the BioSprint 96 DNA Plant Kit and BioSprint 96 DNA Plant protocol. Purified DNA was eluted in 200 µl Buffer AE. Eluates (5 µl) were visualized on a 0.8% agarose gel. M: markers (1 kb ladder).
High PCR performance of purified DNA.
Genomic DNA was purified from 50 mg samples of fresh leaf tissue from [A] maize, [B] arabidopsis, and [C] rose using the BioSprint 96 DNA Plant Kit and BioSprint 96 DNA Plant protocol. DNA was eluted in 200 µl Buffer AE. Amplification (20 µl reaction volume) was carried out using the trnL PCR system (amplifying an intergenic region of a tRNA coding gene in the chloroplast genome) with 5 µl, 0.5 µl, or 0.05 µl purified DNA from 2 maize samples and 3 arabidopsis samples and with 0.5 µl or 0.05 µl purified DNA from 3 rose samples. M: markers (1 kb ladder).
The BioSprint 96 DNA Plant Kit enables purification of DNA from a wide range of plant species (see table).
The BioSprint 96 DNA Plant Kit is used with the BioSprint 96. The kit uses magnetic-particle technology for rapid DNA purification from plant material. The BioSprint 96 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from well to well. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
The BioSprint 96 DNA Plant Kit enables purification of total DNA from a wide variety of plant species, including difficult plant material. Fresh, frozen, or lyophilized starting material is mechanically disrupted to give a fine powder using a homogenizer such as the TissueLyser LT or TissueLyser II. The powder is resuspended in lysis buffer, thoroughly mixed, and then sedimented by a short centrifugation step. Cleared plant lysates are transferred to an S-Block and loaded into the instrument, which then performs all steps until pure DNA is obtained (see flowchart "BioSprint DNA Plant procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.
The BioSprint 96 DNA Plant Kit purifies DNA for use in a range of downstream applications, including:
PCR, real-time PCR, genotyping
For automated processing
BioSprint 96 workstation
5-tube strip, S-Block
Main sample type
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein