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artus Enterovirus LC RT-PCR Kit RUO

For quantitative detection of enterovirus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 3.2 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus Enterovirus LC RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation.
Cat No./ID: 4510003
artus Enterovirus LC RT-PCR Kit (24) RUO
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4510005
artus Enterovirus LC RT-PCR Kit (96) RUO
For 96 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
0
Reliable quantitation of enterovirus load.
Detection of the quantitation standards (Enterovirus LC QS 1–4) on LightCycler 1.1/1.2/1.5 instruments using the artus Enterovirus LC RT-PCR Kit. NTC: no template control.
Performance

To ensure sensitivity, the artus Enterovirus LC RT-PCR Kit has been optimized to detect low numbers of enterovirus RNA. The analytical sensitivity of the kit is 3.2 copies/µl in the PCR.

Performance of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Analytical sensitivity 3.2 copies/µl in the PCR

Principle

The artus Enterovirus LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the enterovirus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus Enterovirus LC RT-PCR Kit provides 4 enterovirus quantitation standards (see figure "Reliable quantitation of enterovirus load". Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical enterovirus RT-PCR is not reduced.

Specifications of artus Enterovirus LC RT-PCR Kit
Kitartus Enterovirus LC RT-PCR Kit
Sample type Cerebral spinal fluid (CSF)
Amplicon 114 bp region of the enterovirus genome

Procedure

The artus Enterovirus LC RT-PCR Kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

Applications
The artus Enterovirus LC RT-PCR Kit enables rapid and sensitive detection of enterovirus RNA from cerebrospinal fluid (CSF) with accurate quantification. The kit is available for use on the Lightcycler 1.1/1.2/1.5 Instruments.
Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp Viral RNA Mini Kit
RUO/CE/ASR RUO
Sample type Cerebrospinal fluid (CSF)
Thermal cycler LightCycler
What detected Enterovirus RNA
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