For sensitive detection of His-tagged proteins with an RGS·His epitope
Highly specific detection of C-terminal, N-terminal, and internal His tags
Highly sensitive and specific detection of RGS·His epitopes
Monoclonal mouse Anti·His Antibodies, like the RGS·His Antibody, BSA-free, are used to detect recombinant protein carrying His tags. RGS·His Antibody is one of three variants, each of which detects a different His tag epitope. Like all QIAGEN mouse monoclonal antibodies, RGS·His Antibody, BSA-free, is prepared under serum-free conditions, which guarantees the absence of viruses, mycoplasma, and contaminating immunoglobulins.
100 μg mouse anti·RGS(H)4 (lyophilized, BSA-free, for 1000 ml working solution)
Performance of RGS·His Antibody, BSA-free
N-terminal His tags (containing the epitope RGSHHHH)
Sensitivity in dot blots:*
Sensitivity in western blots:*
Negligible with crude E. coli, yeast, mammalian, or insect cell lysates
* Detection using chromogenic substrate. Sensitivity may vary with different proteins.
Many different proteins have been detected, via their His tags, using the QIAexpress
Anti·His Antibodies (see table). For the sensitivity of the three Anti·His Antibodies
in detecting this panel of proteins see the figure
Sensitivity of QIAexpress Anti·His Antibodies)
G DNA Polymerase
All QIAexpress mouse monoclonal antibodies are produced under conditions that guarantee the highest purity and activity. Hybridoma cell culture is carried out under serum-free conditions, ensuring that the preparations are free of viruses, mycoplasma, and contaminating immunoglobulins. Purification by adsorption chromatography is performed entirely at physiological pH, yielding preparations with the highest proportion of native antibody.
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
QIAexpress Detection Systems, including RGS·His Antibody, BSA-free, allow efficient detection of His-tagged proteins and are ideal for many applications, including:
Colony, dot, and western blotting procedures
Screening for positive expression clones
Monitoring expression levels and stability of His-tagged proteins
Immunoprecipitation and ELISA
Immunocytochemistry and immunohistochemistry
Secondary antibody required
Sensitivity in dot blots -chemiluminescent substrate-
Sensitivity in dot blots -chromogenic substrate-
Sensitivity in western blots - Chemiluminescent substrate
Sensitivity in western blots -chromogenic substrate-