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QIAamp UCP Pathogen Mini Kit

For microbial DNA purification from whole blood, swabs, cultures, and body fluids

Features

  • Purification of pathogen DNA from small volumes
  • Efficient mechanical disruption of cells using Pathogen Lysis Tubes
  • Pretreatment and simplified purification vacuum and spin protocols
  • Ultraclean production (UCP) reagents and consumables
  • Highly suitable for deep sequencing
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QIAamp UCP Pathogen Mini Kit

Cat. No. / ID: 50214

50 QIAamp UCP Mini Columns, Collection Tubes (2 ml), Tube Extenders (20 ml), Elution Tubes, VacConnectors, Buffers, and Proteinase K
The QIAamp UCP Pathogen Mini Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The QIAamp UCP Pathogen Mini Kit is for microbial DNA purification from small volumes of whole blood, swabs, cultures, and body fluids.

Performance

The QIAamp UCP Pathogen Mini Kit, when used with Pathogen Lysis Tubes, provides efficient cell lysis, with high yields of pathogen DNA. Pathogen Lysis Tubes with bead types S or L are available as accessories.

The QIAamp UCP Pathogen Mini Kit purifies microbial DNA for use in downstream applications. Ultraclean production (UCP) reagents and consumables provide high-quality purified DNA for your downstream applications. The QIAamp UCP Mini Columns and all buffers within the QIAamp UCP Pathogen Mini Kit undergo DNA decontamination processes and are certified to be free of contaminating microbial DNA at the time of delivery.

Principle

The QIAamp UCP Pathogen Mini Kit enables purification of microbial DNA using tailored sample pretreatement protocols with simplified sample prep protocols (spin protocol or vacuum protocol). Pretreatment protocols include mechanical disruption using Pathogen Lysis tubes (available as accessories) for maximal lysis efficiency for cells of gram-positive bacteria, yeast, and other fungi. Using the QIAvac 24 Plus with the QIAamp UCP Pathogen Mini Kit enables starting samples of up to 400 µl and flexible elution volumes from 50 to 200 µl. Multiple samples may be processed simultaneously, providing pure nucleic acids in less than 2 hours per 24 samples.

Procedure

The QIAamp UCP Pathogen Mini Kit uses tailored sample pretreatment protocols with sample prep protocols. Using Pathogen Lysis Tubes for mechanical disruption provides efficient lysis for cells of Gram-positive or Gram-negative bacteria, as well as yeast, and other fungi.

Applications

The QIAamp UCP Pathogen Mini Kit can be used for purification of bacterial and fungal nucleic acids from a variety of sources, including 

  • Culture
  • Whole blood 
  • Swabs 
  • Biological fluids, like urine 

Resources

Kit Handbooks (2)
全血、スワブ、培養液、体液からの微生物DNA 精製
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728