Oligotex mRNA Kits contain spin columns and all necessary reagents and buffers for isolation of pure poly A+ mRNA. The Oligotex low-throughput mRNA purification procedure enables isolation of pure poly A+ mRNA from total RNA in as little as 30 minutes, with >90% recovery. Isolation of poly A+ mRNA from total RNA usually provides slightly greater enrichment than direct isolation from cells or tissues, since there is no risk of interference by other cellular components.
Oligotex resin consists of polystyrene–latex particles of uniform size (1.1 µm diameter) and a perfect spherical shape (see figure " Oligotex particles and oligo-dT cellulose") with dC10T30 oligonucleotides covalently linked to the surface. The size, composition, and surface structure of Oligotex oligo-dT beads have been optimized for uniform dispersion and minimal centrifugation time. The particles form a stable suspension that provides a large surface area for rapid and efficient binding of polyadenylic acids.
The high capacity and accuracy of hybridization provides superior purification of poly A+ mRNA through fast and efficient hybridization. mRNA recoveries are consistently greater than 90%. The Oligotex bead-based mRNA purification procedure minimizes loss of poly A+ RNA, eliminates the risk of degradation by RNases, and requires minimal hands-on time. This makes it ideal for simultaneous handling of multiple samples. Poly A+ mRNA is immediately ready for all standard applications, as well as for sensitive applications such as expression array, differential display, cDNA library construction, microinjection, and SAGE technology.