Total RNA was purified from Jurkat cells (105, 104, 103, and 102) using the RNeasy Plus 96 Kit. Real-time RT-PCR analysis of the β-actin transcript was performed using a primer–probe set that could amplify and detect both mRNA and genomic DNA sequences. The flat amplification plot for the no RT control (–RT) indicates the absence of genomic DNA.
Total RNA was purified from the indicated cell lines using the RNeasy Plus 96 Kit and analyzed on the Agilent 2100 Bioanalyzer. The RIN values were close to 10, indicating the high integrity of the purified RNA.
Total RNA was purified from 10-fold serial dilutions of Jurkat cells (106 to 102 cells) using the RNeasy Plus 96 Kit. Real-time RT-PCR analysis of the β-actin transcript was performed. The linear graph of CT values against cell dilution indicates reliable RNA purification over a wide range of amounts of starting material (R2 = 0.9985).