QIAexpress Type IV Kit
For high-level expression and one-step purification of N-terminally His-tagged proteins
The QIAexpress System provides materials for expression, purification, detection, and assay of His-tagged proteins.
The QIAexpress Type IV Kit gives high expression, up to 50% of total cellular protein.
QIAexpress pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli. Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. The pQE vectors (see figure pQE Vectors and table) enable placement of the His tag at either the N- or C-terminus of the recombinant protein.
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. Expression is induced by addition of IPTG. QIAexpress Type IV Kit constructs can be transformed into E. coli, used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells.
The QIAexpress Expression System provides high-level expression of proteins suitable for many applications, including:
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