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Ni-NTA HRP Conjugate

For simple, direct detection of His-tagged proteins without secondary antibodies

  • Direct detection of His-tagged proteins
  • Streamlined detection, eliminating the need for antibodies
  • Sensitive detection using small quantities of conjugate

Ni-NTA HRP Conjugate is used to detect recombinant proteins carrying His tags without the need for antibodies. The Ni-NTA moiety binds to a protein's His tag and the complex is localized using chromogenic substrates that react with conjugated horseradish peroxidase (HRP).

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Cat No./ID: 34530
Ni-NTA HRP Conjugate

Horseradish peroxidase-conjugated Ni-NTA (lyophilized, for 500 ml working solutions)

This product will be discontinued January 31, 2019. Penta·His HRP Conjugate Kit, cat. no. 34460, replaces this product.

The Ni-NTA HRP Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Performance of Ni-NTA HRP Conjugate
Detection: His-tagged proteins with as few as two exposed histidine residues
Sensitivity in dot blots:* 1–2 ng
Sensitivity in western blots:* 2–5 ng
Working concentration: 1/1000 dilution of stock solution
Cross-reactivity: High concentrations of proteins with metal-binding motifs, e.g., the 31 kDa molecular weight standard bovine carbonic anhydrase, a metalloenzyme binding 1 molecule of zinc per molecule of protein
Form: Lyophilized
Working solution: 500 ml

* Detection using chromogenic substrate. Sensitivity may vary with different proteins


Ni-NTA HRP Conjugate consists of Ni-NTA coupled to horseradish peroxidase (HRP). It can be used for direct, straightforward detection of any recombinant protein with an accessible His tag. It eliminates the need for secondary antibodies, thereby saving time in detection procedures. Ni-NTA HRP Conjugate is recommended for use with chromogenic substrates, and for E. coli expression systems. The use of chemiluminescent substrates with Ni-NTA conjugates is not recommended.


Ni-NTA HRP Conjugates are recommended for detection of proteins in E.coli by western, dot, and colony blotting procedures.


Epitope detected Histidine residued epitope
Format Bead based
Instrument Manual
Ligand on bead or plate Ligand on bead
Tag 6xHis tag

Product Resources

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Kit Handbooks (1)
For Anti·His Antibodies, Anti·His HRP Conjugates, Penta·His™ Alexa Fluor® Conjugates, Penta·His Biotin Conjugate, Ni-NTA Conjugates, Tag·100™ Antibody, Streptavidin–R-PE, 6xHis Protein Ladder, Ni-NTA HisSorb™ Strips and Plates 
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Safety Data Sheets (2)
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