For highly sensitive and specific detection of GST-tagged proteins
GST-tag Antibody allows immunologic applications such as western blot, enzyme-linked immunosorbent assay (ELISA), and immunoprecipitation. The antibody is highly sensitive and allows detection of amounts of GST protein as low as 0.5 ng.
The highly specific and sensitive GST-tag Antibody enables detection of even low amounts of GST-tagged proteins, up to 0.5 ng in western blots (see figure "Sensitivity and specificity of GST-tag Antibody").
The GST-tag is a versatile tool for purification and detection of proteins. The monoclonal antobodies have no cross-reactivity with proteins from various other species, giving highly sensitive and specific detection (see figure "Sensitivity and specificity of GST-tag Antibody").
Proteins expressed with a GST-tag are readily isolated from a lysate using Glutathione HiCap Matrix. The purity of the GST-tagged protein can be confirmed by western blotting. After transfer of the isolated protein to the membrane, this is incubated with a blocking solution to minimize non-specific background staining. The membrane is subsequently incubated with GST-tag Antibody diluted appropriately, followed by secondary antibody specific for the GST-tag Antibody. The secondary antibody is conjugated to allow chromogenic or chemiluminescent detection (see figure "Sensitivity and specificity of GST-tag Antibody").
GST-tag Antibody allows immunologic applications, such as:
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