Factor Xa Protease
For endoproteolytic removal of short 6xHis affinity tags from 6xHis-tagged recombinant proteins
Factor Xa Protease is a site-specific endoprotease that preferentially cleaves the C-terminal peptide bond of the recognition sequence Ile-Glu-Gly-Arg. Therefore, Factor Xa Protease efficiently cleaves 6xHis-tagged proteins expressed by the pQE-30 Xa vector as the 6xHis tag precedes the recognition sequence, giving protein free of vector-encoded amino acids. After cleavage of the purified protein, Factor Xa Protease is removed in a batch procedure using Xa Removal Resin.