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artus WNV LC RT-PCR Kit CE

For quantitative detection of West Nile virus RNA using real-time PCR
  • Compliance with EU IVD Directive 98/79/EC
  • High reliability using the internal control
  • Highly sensitive detection of as few as 2.4 copies/µl
  • Accurate quantitation using the 4 standards supplied
The artus WNV LC PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation for in vitro diagnostic use.

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Cat No./ID: 4509063
artus WNV LC RT-PCR Kit (24) CE
For 24 reactions on the LightCycler 1.1/1.2/1.5 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
The artus WNV LC RT-PCR Kit CE is intended for in vitro diagnostic use in Europe.

Product Details

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Reliable quantitation of WNV load.
Detection of the quantitation standards (WNV LC QS 1–4) on the LightCycler instrument using the artus WNV LC RT-PCR Kit. NTC: no template control.
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CE-IVD-marked.
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In vitro diagnostic medical device.
Performance

To ensure highest sensitivity, the artus WNV LC RT-PCR Kit has been optimized to detect low numbers of West Nile virus RNA. The analytical sensitivity of the artus WNV LC RT-PCR Kit is 2.4 copies/µl in the PCR on the LightCycler 1.1/1.2/1.5 Instruments.

Performance characteristics of artus WNV LC RT-PCR Kit
Kitartus WNV LC RT-PCR Kit
Validated sample types Serum, plasma, or cerebral spinal fluid (CSF)
Analytical sensitivity 2.4 copies/µl in the PCR

Principle

The artus WNV LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the West Nile virus genome using real-time PCR. The kit provides high levels of specificity, sensitivity, and reproducibility.

Each artus WNV LC RT-PCR Kit provides 4 quantitation standards (see figure "Reliable quantitation of WNV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical WNV RT-PCR is not reduced.

Specifications of artus WNV LC RT-PCR Kit
Kitartus WNV LC RT-PCR Kit
Validated sample types Serum, plasma, or cerebral spinal fluid (CSF)
Amplicon 72 bp region of the West Nile virus genome

Procedure

The artus WNV LC RT-PCR Kit provides all necessary reagents optimized for reliable West Nile virus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, the QIAamp Viral RNA Mini Kit is to be used.
Applications
The artus WNV LC RT-PCR Kit enables rapid and sensitive detection of West Nile virus RNA from serum, plasma, and cerebral spinal fluid (CSF) with accurate quantification. The kit is available for use on the LightCycler 1.1/1.2/1.5 Instruments.

Specifications

Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp Viral RNA Mini Kit
RUO/CE/ASR CE
Sample type Serum, plasma, cerebrospinal fluid (CSF)
Thermal cycler LightCycler 1.1/1.2/1.5
What detected West Nile virus RNA

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