text.skipToContent text.skipToNavigation

Qproteome Total Glycoprotein Kit

Glycosylated proteins을 분리할 수 있습니다
  • Glycan moieties의 구조에 따라 매우 특이적으로 glycoprotein 분리
  • 다른 조건에서 배양된 세포로에서 glycoproteins profiling
  • Glycoprotein을 정확하고 포괄적으로 관찰할 수 있는 lectin column 선택 가능

The kit contains lectin-resin filled spin columns, buffers, and reagents for the isolation of glycoproteins from cell lysate or serum samples.

Spin Column Adapter Rings are required for automation of this kit on the QIAcube.

Cat No./ID: 37541
Qproteome Total Glycoprotein Kit
Order Product
For 6 total glycoprotein preps: Buffers, Lectin Spin Columns (6), Detergent Solution, Protease Inhibitor Solution, Collection Tubes (6 x 2 ml)
The Qproteome Glycoprotein Kit allows highly specific fractionation of proteins based on their glycan moieties (see figure "Highly specific glycoprotein fractionation").

Glycosylated proteins in the sample bind to lectins immobilized on the resin in the spin columns. After washing, proteins are eluted in two stages by sequential addition of buffers containing sugars which compete with bound proteins for the lectin binding sites.

The sample is diluted and applied to the spin column (see figure "Glycoprotein fractionation procedure"). After a short incubation, non-glycosylated proteins are removed by centrifugation. Glycosylated proteins are eluted by application of elution buffer and centrifugation.

Glycosylation plays a vital role in a wide range of cellular processes such as cell adhesion and signaling, stabilization of protein structure and function, protein trafficking and sorting, and oncogenesis. Several diseases (e.g., rheumatoid arthritis, muscular dystrophy) may be caused by a defect in protein glycosylation. The Qproteome Glycoprotein Fractionation Kit offers highly specific separation of glycoproteins according to the structure of their glycan moieties, and permits profiling of glycoproteins in cells grown under different conditions. Purified glycoproteins are suitable for direct analysis using blotting procedures, 2D-PAGE, or mass spectrometry methods.

Applications SDS-PAGE, mass spectrometry
Binding capacity/yield 30–150 µg
For glycoproteins: which type of glycoproteins Glycoproteins with different structures of their glycan moieties
Fractions isolated One fraction
Protein with post-translational modification Glycosylation
Sample size 50 µl or 1 x 10e7 cells
Start material Serum, cell cultures

Customers who bought these products also bought