Attractene Transfection Reagent

For efficient DNA transfection and DNA–siRNA/miRNA cotransfection

Features

  • Highly efficient DNA transfection with extremely low cytotoxicity
  • Rapid Fast-Forward Protocol
  • Reagent of choice for all adherent cells and sensitive cells
  • Ideal for cotransfection and vector-based RNAi
  • Free of animal-derived components
Attractene Transfection Reagent (1 ml)

Cat. No. / ID: 301005

Attractene Transfection Reagent for up to 660 transfections in 24-well plates
₩518,000.00
Quantity
1 ml
4 ml ( 4 x 1ml)
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The Attractene Transfection Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

Attractene Transfection Reagent represents the next generation in lipid technology, ensuring highly efficient DNA transfection of eukaryotic cells. Attractene Reagent is a nonliposomal lipid that enables transfection of all adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116, and some suspension cell types (Jurkat, K562). It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors.

Please note: The product 1051561: Attractene Transfection Reagent (0.1 ml) is phasing-out by December 31, 2015 and available only while stock lasts. Please contact your customer care department. Please use product 301005: Attractene Transfection Reagent (1 ml) as alternative.

Performance

Attractene Reagent provided higher transfection efficiency than alternative reagents when transfection was performed without any optimization following the recommended conditions provided in the handbooks (see figure " Attractene Reagent outperforms alternative reagents"). Cytotoxicity must be kept to a minimum to ensure that results are due to the nucleic acid transfected and not the transfection process itself. Attractene Reagent ensures exceptionally low cytotoxicity (see figure " Healthy cells after transfection using Attractene Reagent"). Using Attractene Reagent ensures that transfection of shRNA (short-hairpin RNA) vectors for gene silencing experiments can be achieved with high efficiency (see figure " Effective knockdown after shRNA vector transfection").

 

 

See figures

Principle

Attractene Reagent is a nonliposomal lipid which enables highly efficient DNA transfection of some suspension cell types (Jurkat, K562), and adherent cells, including difficult-to-transfect cell types such as HaCaT, MonoMac6, and HCT116. Attractene Reagent consistently provides higher transfection efficiency than alternative reagents under a range of experimental conditions. This feature allows flexible handling and ease of use for many experimental setups such as using automated platforms and transfection at high- or low-throughput levels. It is also highly suitable for cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Using Attractene Reagent ensures exceptionally low cytotoxicity, which is critical to successful transfection experiments.

Procedure

Attractene Transfection Reagent is highly suited for rapid fast-forward DNA transfection. In Fast-Forward Protocols, cells are seeded and transfected on the same day (see figure " Flexible, rapid Fast-Forward Protocols"). This is quicker, saves labor, and increases experimental flexibility compared to protocols where cells are seeded the day before transfection.

Cell type-specific protocols at the TransFect Protocol Database

In addition to the protocols provided in the Attractene Transfection Reagent Handbook, you can find protocols to suit your cell type and plate/dish format using QIAGEN's TransFect Protocol Database. The database provides exactly the protocol needed, saving time and effort. Simply enter the cell type and nucleic acid of interest to receive a QIAGEN transfection protocol to print out or download in convenient PDF format. Use of the TransFect Protocol Database is free of charge and no registration is required.

 

See figures

Applications

Attractene Transfection Reagent can be used for a range of transfection applications, including transient or stable transfection, or cotransfection of multiple DNA constructs, and cotransfection of DNA with siRNA or miRNA mimics or inhibitors. Attractene Transfection Reagent can be used in:

  • Functional genomics
  • High-throughput DNA transfection
  • Gene silencing

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsPlasmid transfection, shRNA vector transfection, protein overexpression, reporter studies, RNAi experiments
Number of possible transfectionsup to 660 transfections in 24-well plates / 1 ml reagent
Cell typeAll cell types including sensitive and difficult to transfect cells
Nucleic acidDNA
Transfection typeTransient transfection, stable transfection, plasmid co-transfection
TechnologyCationic lipid based transfection reagent
ControlsNot included
FeaturesHighly efficient transfection with lowest toxicity. Rapid fast forward transfection protocol. Free of animal-derived components. Transfection in the presence of serum.

Resources

Kit Handbooks (1)
For efficient DNA transfection of a broad range of cell lines, including sensitive cell types
Brochures & Guides (2)
Brochure detailing reagents for efficient and robust DNA and RNA transfection.

FAQ

Which transfection reagent is recommended for transfection of the miScript Target Protectors?
HiPerfect Transfection Reagent is recommended for the transfection of the miScript Target Protector alone. Attractene Transfection Reagent is recommended for DNA/oligo co-transfection.
FAQ ID -2252
Do you have a protocol for co-transfection of adherent cells with siRNA and plasmid DNA?

Yes, please follow the Supplementary Protocol 'Guidelines for co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent' (TFP21).

 In addition, we have also the following Supplementary Protocol for the Attractene Transfection Reagent 'Fast-Forward cotransfection of adherent cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent'.

FAQ ID -969
What is the most reliable transfection reagent for delivering shRNA plasmids and siRNA to cells in culture?

HiPerFect Transfection Reagent is optimized for siRNA transfections and enables effective siRNA uptake and efficient release of siRNA inside cells, resulting in high gene knockdown even when using low siRNA concentrations. For high-throughput siRNA screenings HiPerFect HTS Reagent is a fast and effective newcomer specifically designed to focus on robustness and cost efficiency. For the transfection of shRNA.

Attractene Transfection Reagent should be used for the transfection of shRNA (short-hairpin RNA) vectors for gene silencing experiments to achieve high efficiency. Ease and flexibility of handling enables preparation and storage of transfection complexes making Attractene Reagent suitable for use with automated systems.

http://www.sabiosciences.com/reversetransfection.php

FAQ ID -2777
Can I use RNAiFect for co-transfection of plasmid DNA and siRNA?

No, we do not recommend to use RNAiFect for the transfection of plasmid DNA. The RNAiFect Kit does not contain Enhancer which is necessary to form condensed complexes before transfection of DNA into the cell. TransMessenger Transfection Reagent would be the right choice as it allows co-transfection of siRNA and plasmid DNA. Access the protocol via the following link: Co-transfection of adherent cells with siRNA and plasmid DNA using TransMessenger Transfection Reagent

Alternatively, you can use the Attractene Transfection Reagent with the QIAGEN Protocol "Fast -Forward cotransfection of adhernet cells with siRNA and DNA in 24-well plates using Attractene Transfection Reagent".

FAQ ID -515
What transfection method should I use with the SureSilencing shRNA Plasmids?
We recommend using Attractene Transfection Reagent. Use either the GFP-based SureSilencing shRNA plasmids or another plasmid system that has an easy screening marker that allows you to count cells, such as GFP or beta-galactosidase. Ease and flexibility of handling enables preparation and storage of transfection complexes making Attractene Reagent suitable for use with automated systems.
FAQ ID -2901