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HiSpeed Plasmid Maxi Kit

 Plasmid 혹은 cosmid DNA를 750 ug까지 초고속으로 분리 정제할 수 있습니다
  • Cell harvest에서 transfection grade plasmid DNA까지 60분 소요
  • 원심분리 없이 lysate clearing과 isopropanol precipitation
  • Precipitation 동안 pellet 손실 위험 없음
  • Up to 750 ug yield of high-copy plasmid DNA
  • LyseBlue for optimum lysis and maximum DNA yield

HiSpeed Plasmid Kits provide fast, large-scale, anion-exchange-based plasmid DNA preparation without centrifugation. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation and is suitable for transfection-grade applications.

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Cat No./ID: 12643
HiSpeed Plasmid Midi Kit (25)
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25 HiSpeed Midi Tips, 25 QIAfilter Midi Cartridges, 25 QIAprecipitator Midi Modules plus Syringes, Reagents, Buffers
Cat No./ID: 12662
HiSpeed Plasmid Maxi Kit (10)
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10 HiSpeed Maxi Tips, 10 QIAfilter Maxi Cartridges, 10 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
Cat No./ID: 12663
HiSpeed Plasmid Maxi Kit (25)
Order Product
25 HiSpeed Maxi Tips, 25 QIAfilter Maxi Cartridges, 25 QIAprecipitator Maxi Modules plus Syringes, Reagents, Buffers
HiSpeed Plasmid Maxi Kit are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


Product Details


HiSpeed Plasmid Kits provide QIAfilter Cartridges, HiSpeed Tips, and QIAprecipitator modules for fast large-scale plasmid preparation. Syringe-format QIAfilter and QIAprecipitator modules replace centrifugation steps in the classic anion-exchange procedure, making plasmid purification faster and more convenient. Up to 750 µg high-copy plasmid DNA can be purified from 150–250 ml culture. (Culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium.) HiSpeed Tip design allows a very high flow rate, permitting DNA binding, washing, and elution steps for plasmid purification to proceed faster.

The unique anion-exchange resin in HiSpeed Tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation.


QIAfilter cartridges (see figure "QIAfilter Cartridge") are special filter units designed to replace the centrifugation step following alkaline lysis of bacterial cells. QIAfilter Cartridges completely remove SDS precipitates and clear bacterial lysates in a fraction of the time needed for centrifugation. Prepacked HiSpeed Tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation.

The unique QIAprecipitator module (see figure "QIAprecipitator module") replaces the centrifugation step traditionally used to collect isopropanol-precipitated DNA following purification. The QIAprecipitator module traps the precipitated DNA, while the isopropanol-buffer mixture flows through. The DNA is then simply eluted from the QIAprecipitator into a microcentrifuge tube with TE buffer or water. This unique module also eliminates the risk of pellet loss, which can occur during decanting of the supernatant following centrifugation.


Neutralized bacterial lysates are incubated in the QIAfilter Cartridge and cleared in seconds by filtration. The filtrate is applied to a HiSpeed tip for plasmid DNA purification (see flowchart "QIAGEN Plasmid Kit procedures"). Eluted DNA is mixed with isopropanol and applied to the QIAprecipitator module using the syringe provided. The concentrated and desalted DNA is then eluted from the QIAprecipitator directly into a microcentrifuge tube with TE buffer or water. 


DNA purified with HiSpeed Plasmid Kits yields excellent results in all applications, from cloning and sequencing to transfection and plasmid-mediated gene silencing.

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