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RNeasy 96 BioRobot 9604 Kit

 BioRobot 9604 또는 BioRobot 3000 workstation을 이용하여 RNA를 Automated high-throughput으로 minipreps할 수 있는 kit입니다
  • BioRobot 9604 또는 BioRobot 3000에 적합
  • 빠르고 편리한 샘플 처리
  • Organic extraction 또는 precipitation 불필요
  • 10–500,000 cells에서의 재현성 있는 yield
  • 다양한 application을 위한 양질의 RNA

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Cat No./ID: 967142
RNeasy 96 BioRobot 9604 Kit (12)
For 12 x 96 total and cytoplasmic RNA preps on the BioRobot 9604 or BioRobot 3000: 12 RNeasy 96 Plates, Elution Microtubes CL, Caps, S-Blocks, AirPore Tape Sheets, RNase-Free Reagents and Buffers
The RNeasy 96 BioRobot 9604 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Product Details

9
RNeasy 96-well plate.
 
9
RT-PCR of RNA from as few as 100 cells.
 
9
Repeatability of automated RNA purification.
 
9
Reproducible yields of high-quality RNA.
 
9
High-quality RNA for sensitive analysis of a low-copy transcript.
 
Performance
The RNeasy 96 system provides fast and reproducible total RNA purification for high-throughput gene expression profiling. The RNA is suitable for sensitive applications such as quantitative, real-time RT-PCR and microarray analysis (see figure "High-quality RNA for sensitive analysis of a low-copy transcript"). Sample sizes range from 10 to 5 x 105 cells (see figure "RT-PCR of RNA from as few as 100 cells"), and high-quality RNA can be purified from large numbers of samples (see figure "Reproducible yields of high-quality RNA"). Individual variations are low throughout the entire purification process; TaqMan threshold-cycle values are easily achieved at the end of the process with a coefficient of variation (CV) less than 3% using the QuantiTect Probe RT-PCR Kit (see figure "Repeatability of automated RNA purification").
Principle

The RNeasy 96 system provides a standardized, fast, reliable method for isolation of high-quality RNA from large numbers of samples (see table). The simple procedure is the key to the speed and efficiency of RNeasy 96 technology. Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate (see figure "RNeasy 96-well plate"), RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes suitable for long-term storage and is ready to use for any experiment. Since the RNeasy procedure enriches for RNA species >200 nt, RNA yield does not include 5S rRNA, tRNAs, or other low-molecular-weight RNAs.

Specifications of the RNeasy 96 BioRobot 9604 Kit
SpecificationDetails
Format RNeasy 96-well plates with 1.2 ml collection microtubes
Sample source Animal or human cells
Sample size 10–5 x 105 cells
Binding capacity Up to 100 µg RNA per well
Yield* from 1 x 105 cells 

HeLa: 1.6 µg
LMH: 1.3 µg
COS-7: 3.1 µg
Huh7: 2.0 µg
Jurkat: 1.4 µg

Elution volume 45–140 µl
* Amounts can vary due to developmental stage, growth conditions used, etc.

Procedure

The simple procedure is the key to the speed and efficiency of RNeasy 96 technology. Cells can be grown and directly lysed in 96-well cell-culture dishes. After transfer of the lysates to the wells of the RNeasy 96 plate, RNA binds to the silica membrane in each well, and contaminants are washed away. Pure RNA is then eluted in RNase-free water into individual collection tubes suitable for long-term storage and is ready to use for any experiment. The RNeasy 96 BioRobot 9604 Kit includes protocols for total RNA isolation and cytoplasmic RNA isolation. RNA isolation is automated on the BioRobot 9604. Depending on the downstream application, manual RNA purification using the RNeasy 96 Kit can be performed on the QIAvac 96 vacuum manifold and/or  the 96-Well-Plate Centrifugation System.

Fully automated RNA isolation is possible on the BioRobot 8000 workstation or BioRobot Universal System using the RNeasy 96 BioRobot 8000 Kit.

Applications

The RNeasy 96 BioRobot 9604 Kit  and the RNeasy 96 Kits consistently provide the highest-quality RNA for:

  • High-throughput RNA applications in gene expression analysis
  • Sensitive applications such as quantitative, real-time RT-PCR
  • Pharmacological and toxicological research
  • High-throughput drug screening 
  • Biomedical research and molecular diagnostics

Specifications

Features
Specifications
Applications PCR, real-time PCR, microarray
Elution volume 45–140 µl
For automated processing BioRobot 9604 or BioRobot 3000 Workstation
Format 96-well plate
Main sample type Cultured cells
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein RNA
Sample amount 5 x 10e5
Technology Silica technology
Yield 1.3 –3.1 µg

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FAQs (17)
Kit Handbooks (1)
For automated RNA isolation from animal and human cells
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