REPLI-g Single Cell DNA Library Kit
Choose from a wide range of REPLI-g Kits
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Dedicated buffers and reagents have been specifically developed to deliver high yields of DNA from a wide range of sample types, including single cells, limited tissue material, FFPE samples, mitochondrial DNA and purified DNA, with complete sequence representation and unbiased amplification.

 

REPLI-g Kit specifications
Kit Application  Starting material Kit output  Yield (µg/reaction) Reaction time (h) Hands-on time (min)
REPLI-g Single Cell Kit
Single-cell whole genome amplification
 Single cells,
1–1000 cells, tissue, purified gDNA (1–10 ng)
Whole genome amplified DNA for analysis by cGH array, microarray, qPCR or NGS with the highest sensitivity
40 µg
8–16 h
15 min
REPLI-g WTA Single Cell Kit
Single-cell whole transcriptome amplification
Single cells (mammalian),
1–1000 cells, purified RNA (10 pg – 100 ng)
Whole transcriptome amplified cDNA for subsequent analysis by microarray, qPCR or NGS with the highest sensitivity
20 µg cDNA
4 h
20 min
REPLI-g Cell WGA & WTA Kit
Parrallel whole genome and whole transcriptome amplification starting with a few cells
25–1000 mammalian cells
Whole genome amplified DNA and whole transcriptome amplified cDNA
Approx. 20 µg cDNA, approx. 20 µg DNA
4 h
20 min
REPLI-g Mini Kit
Small-sample whole genome amplification




>10 ng gDNA, 0.5 µl blood or cells (600 cells/µl)









Whole genome amplified DNA for subsequent analysis by microarray, cGH array, qPCR, Sanger sequencing or NGS





 10–40 µg
10–16 h
15 min
REPLI-g UltraFast Mini Kit
Small-sample whole genome amplification, with a focus on short time-to-result
7–10 µg
1.5 h
15 min
REPLI-g Midi Kit
Small-sample whole genome amplification, with a focus on high yield
40 µg
8–16 h
15 min
REPLI-g Screening Kit
Small-sample whole genome amplification, with a focus on high sample throughput
8 µg
12–16 h
15 min
REPLI-g FFPE Kit
Whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
FFPE tissue section (1 cm diameter, 10–10 µm thick); 100 ng gDNA from FFPE tissue
Standard yield: ≤10; high-yield: ≤40
Standard yield: 4 h;
high yield: 10 h
40 min
REPLI-g Mitochondrial DNA Kit
Whole genome amplification of mitochondrial DNA
>1 ng purified gDNA
8 µg
8 h
15 min


注目製品
11
REPLI-g Single Cell DNA Library Kit
For DNA library construction from single cells for Illumina sequencing applications
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REPLI-g Single Cell RNA Library Kit
For RNA library construction from single cells for Illumina sequencing applications 
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細胞や限られた量のサンプルから、全ゲノムおよび全トランスクリプトームを同時増幅
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シングルセル由来のトータルRNAやmRNAの全トランスクリプトーム増幅
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REPLI-g Single Cell Kit (24)
シングルセルあるいは限られたサンプル量からの全ゲノム増幅(WGA)
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微量あるいは貴重なサンプルから全ゲノムを均一に増幅
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微量あるいは貴重なサンプルから全ゲノムを均一に増幅
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S_1323_AppD_REPLI-g0468
微量あるいは貴重なサンプルから全ゲノムを均一かつ迅速に増幅
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微量あるいは貴重なサンプルから全ゲノムをハイスループットで増幅(マニュアルあるいは自動化)
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REPLI-g Mitochondrial DNA Kit
ミトコンドリア全ゲノムの均一な増幅
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ホルマリン固定パラフィン包埋 (FFPE) 組織切片から直接全ゲノムDNA を増幅
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キットハンドブック
9

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詳細を表示
For whole genome amplification from single cells, limited samples, or purified genomic DNA
詳細を表示
For whole transcriptome amplification of total RNA from single cells
詳細を表示
For parallel whole genome amplification and whole transcriptome amplification from cells and limited samples
詳細を表示
For whole genome amplification from purified genomic DNA, blood, and cells
詳細を表示
For fast whole genome amplification from purified genomic DNA, blood, and cells
詳細を表示
For whole genome amplification from purified genomic DNA, blood, and cells
詳細を表示
For direct whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
詳細を表示