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ipsogen BCR-ABL1 mbcr Kit

For quantitative detection of BCR-ABL mbcr e1a2 transcripts using real-time PCR

Features

  • Accurate and sensitive quantitative assay
  • EAC standardized assay for reproducible results
  • Easy workflow with ready-to-use solutions
 

Useful Resources

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ipsogen BCR-ABL1 mbcr Kit (24)

Cat. No. / ID: 670013

For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The ipsogen BCR-ABL1 mbcr Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The kit provides reagents optimized for reliable and sensitive detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA.

Performance

To ensure highest sensitivity, the ipsogen BCR-ABL1 mbcr Kit has been optimized to detect BCR-ABL mbcr p190 e1a2 and ABL transcripts and uses prediluted plasmid standards and primers and probe mixes (see figures  Accurate detection of BCR-ABL mbcr plasmid standards and  Reliable detection of ABL plasmid standards).
See figures

Principle

The ipsogen BCR-ABL1 mbcr Kit is a ready-to-use kit for the detection of BCR-ABL mbcr p190 e1a2 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, in total RNA (see figures  BCR-ABL mbcr fusion gene transcript and  ABL control gene transcript). The kit provides high levels of specificity, sensitivity, and reproducibility. The ipsogen BCR-ABL1 mbcr Kit provides 3 standard dilutions for ABL and 5 standard dilutions for BCR-ABL mbcr. Use of the standards enables accurate quantification of transcripts. The technology used for quantification of BCR-ABL mbcr has been standardized according to EAC (Europe Against Cancer) recommendations and the ipsogen BCR-ABL1 mbcr Kit uses this validated technology to calibrate results.
See figures

Procedure

The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen BCR-ABL1 mbcr Kit allows detection and quantification of BCR-ABL mbcr p190 e1a2 and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.

Applications

The ipsogen BCR-ABL1 mbcr Kit enable sensitive and reliable detection and quantification of BCR-ABL mbcr p190 e1a2 transcripts, relative to ABL control gene expression, for research use only.

Supporting data and figures

Resources

Kit Handbooks (1)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.