Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.
The simple bind-wash-elute procedure of the QuickLyse system.
Plasmid DNA (pSV-β-galactosidase) was purified using the QuickLyse Miniprep Kit (QL), as well as three additional commercially available kits (1–3). Purified plasmid DNA was digested with NdeI and EcoRI restriction enzymes. The reactions were performed in duplicate. M: markers. Ø: uncut plasmid.
Typical sequencing results for plasmid DNA purified using the QuickLyse Miniprep Kit. The pSV-β-galactosidase plasmid was isolated from TOP10 cells and sequenced using the BigDye terminator sequencing chemistry.