Reverse transcription was oligo-dT primed and PCR was carried out using actin-specific primers. [A] Poly A+ mRNA was isolated from 0.25, 0.5, or 1 µg mouse liver total RNA (lanes 1–3), using the Oligotex mRNA Mini Kit. C: negative control. [B] mRNA was isolated from 103 or 104 HeLa cells (lanes 1 and 2), using the Oligotex Direct mRNA Micro Kit. M: 123 bp ladder.
Scanning electron micrographs of Oligotex particles and oligo-dT cellulose at the same magnification. Note the uniform size and shape of Oligotex particles.