The simple Puregene procedure uses a modified salting-out precipitation method for purification of DNA. No mixing or dilution of solutions is necessary, and hands-on time is minimized. The procedure provides convenient stopping points that allow safe, temporary storage of partially purified samples.
DNA was purified from whole blood samples using the Gentra Puregene Blood Kit. Purified DNA was digested with 2 units enzyme per μg DNA for 2 hours at 37°C. Restriction digestion reactions were analyzed by agarose gel electrophoresis on a 0.6% agarose gel. M: Markers.
DNA was purified from replicate whole blood samples using the Gentra Puregene Blood Kit. Purified DNA was analyzed by agarose gel electrophoresis after storage for 18 years at 4°C. M1: Uncut Lambda DNA; M2: Lambda HindIII marker.