PyroMark Q24 Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using the PyroMark Q24

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q24 uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q24 Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q24 Accessories and Reagents are designed specifically for use with the PyroMark Q24 instrument.

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Product	Product no.	Cat. no.	List price:
PyroMark Q24 Vacuum Workstation (220 V) Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template show details	9001518		Inquire	add to cart add to cart
PyroMark Gold Q24 Reagents (5 x 24) Nucleotides, enzyme, and substrate solutions, intended for use with PyroMark Q24 show details	970802		€ 231,00	add to cart add to cart
PyroMark PCR Kit (200) For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water show details	978703		€ 256,00	add to cart add to cart
PyroMark PCR Kit (800) For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water show details	978705		€ 955,00	add to cart add to cart
PyroMark OneStep RT-PCR Kit (50) For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water show details	978801		€ 228,00	add to cart add to cart
PyroMark OneStep RT-PCR Kit (200) For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water show details	978803		€ 677,00	add to cart add to cart
PyroMark Binding Buffer (200 ml) For binding of biotinylated PCR product to Sepharose beads show details	979006		€ 56,90	add to cart add to cart
PyroMark Denaturation Sol. (500 ml) For denaturation of double-stranded PCR product into single-stranded DNA template show details	979007		€ 56,90	add to cart add to cart
PyroMark Wash Buffer (conc., 200 ml) For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA show details	979008		€ 56,90	add to cart add to cart
PyroMark Annealing Buffer For annealing of sequencing primer to DNA template show details	979009		€ 56,90	add to cart add to cart
PyroMark Vacuum Prep Filter Probe (100) Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24 show details	979010		€ 280,00	add to cart add to cart
PyroMark Q24 Plate (100) 24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package show details	979201		€ 233,00	add to cart add to cart
PyroMark Q24 Cartridge (3) Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced show details	979202		€ 435,00	add to cart add to cart
PyroMark Control Oligo For installation check of system show details	979203		€ 320,00	add to cart add to cart
PyroMark Q24 Validation Oligo For performance confirmation of system show details	979204		€ 656,00	add to cart add to cart
PyroMark Q24 Vacuum Prep Troughs (12) Reusable plastic troughs for use with all PyroMark Q24 Vacuum Workstations show details	979206		€ 40,70	add to cart add to cart

PyroMark Q24 Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

FAQs (22)

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	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	View
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	View
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	View
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	View
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	View
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	View
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	View
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	View
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	View
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	View

Brochures & Guides (2)

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	Product Profile - PyroMark Q24 Advanced	Show details
	Pyrosequencing – the synergy of sequencing and quantification	Show details

Kit Handbooks (6)

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	Show details
	(EN) - PyroMark Gold Q24 Reagents For performing Pyrosequencing reactions on the PyroMark Q24	Show details
	(EN) - PyroMark OneStep RT-PCR Handbook	Show details
	(EN) - PyroMark PCR Handbook	Show details
	(EN) - PyroMark Q24 Cartridge Product Sheet	Show details
	(EN) - PyroMark Q24 Validation Oligo Handbook	Show details

User-Developed Protocols (1)

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Show details

White Papers (1)

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Show details

Application Notes (2)

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	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Show details
	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Show details

Quick-Start Protocols (2)

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Show details
	(EN) - PyroMark Q24 Vacuum Workstation Quick-Start Guide	Show details

Safety Data Sheets (13)

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q24 Reagents (5 x 24)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Q24 Cartridge (3)
	MSDS PyroMark Q24 Validation Oligo
	MSDS PyroMark Vacuum Prep Filter Probe (100)
	MSDS PyroMark Wash Buffer (conc., 200 ml)

References

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QIAGEN Multiplex PCR Kit (100)

For 100 x 50 µl multiplex PCR reactions: 2x QIAGEN Multiplex PCR Master Mix (providing a final concentration of 3 mM MgCl₂, 3 x 0.85 ml), 5x Q-Solution (1 x 2.0 ml), RNase-Free Water (2 x 1.7 ml)

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PyroMark Q24 Accessories and Reagents

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