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PyroMark Q24 Accessories and Reagents

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For optimal sample preparation and Pyrosequencing analysis using the PyroMark Q24

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q24 uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q24 Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q24 Accessories and Reagents are designed specifically for use with the PyroMark Q24 instrument.

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Cat No./ID: 9001518

PyroMark Q24 Vacuum Workstation (220 V)

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Vacuum Workstation for preparing 24 samples in parallel from PCR product to single-stranded template

Cat No./ID: 970802

PyroMark Gold Q24 Reagents (5 x 24)

EUR 234,00

Nucleotides, enzyme, and substrate solutions, intended for use with PyroMark Q24

Cat No./ID: 978703

PyroMark PCR Kit (200)

EUR 260,00

For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water

Cat No./ID: 978705

PyroMark PCR Kit (800)

EUR 970,00

For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water

Cat No./ID: 978801

PyroMark OneStep RT-PCR Kit (50)

EUR 231,00

For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water

Cat No./ID: 978803

PyroMark OneStep RT-PCR Kit (200)

EUR 687,00

For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water

Cat No./ID: 979006

PyroMark Binding Buffer (200 ml)

EUR 56,90

For binding of biotinylated PCR product to Sepharose beads

Cat No./ID: 979007

PyroMark Denaturation Sol. (500 ml)

EUR 56,90

For denaturation of double-stranded PCR product into single-stranded DNA template

Cat No./ID: 979008

PyroMark Wash Buffer (conc., 200 ml)

EUR 56,90

For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA

Cat No./ID: 979009

PyroMark Annealing Buffer

EUR 56,90

For annealing of sequencing primer to DNA template

Cat No./ID: 979010

PyroMark Vacuum Prep Filter Probe (100)

EUR 280,00

Reusable filter probes for PyroMark Vacuum Workstation Q96 and Q24

Cat No./ID: 979201

PyroMark Q24 Plate (100)

EUR 233,00

24-well plate for samples, for use with PyroMark Q24 and PyroMark Q24 Advanced; 100 plates in each package

Cat No./ID: 979202

PyroMark Q24 Cartridge (3)

EUR 435,00

Reusable dispensing tool for nucleotide and reagent delivery, for use with PyroMark Q24 and PyroMark Q24 Advanced

Cat No./ID: 979203

PyroMark Control Oligo

EUR 324,00

For installation check of system

Cat No./ID: 979204

PyroMark Q24 Validation Oligo

EUR 665,00

For performance confirmation of system

Cat No./ID: 979206

PyroMark Q24 Vacuum Prep Troughs (12)

EUR 40,70

Reusable plastic troughs for use with all PyroMark Q24 Vacuum Workstations

PyroMark Q24 Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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FAQs (22)

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	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	View
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	View
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	View
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	View
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	View
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	View
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	View
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	View
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	View
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	View

Brochures & Guides (2)

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	Product Profile - PyroMark Q24 Advanced	Show details
	Pyrosequencing – the synergy of sequencing and quantification	Show details

Kit Handbooks (6)

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	Show details
	(EN) - PyroMark Gold Q24 Reagents For performing Pyrosequencing reactions on the PyroMark Q24	Show details
	(EN) - PyroMark OneStep RT-PCR Handbook	Show details
	(EN) - PyroMark PCR Handbook	Show details
	(EN) - PyroMark Q24 Cartridge Product Sheet	Show details
	(EN) - PyroMark Q24 Validation Oligo Handbook	Show details

User-Developed Protocols (1)

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Show details

White Papers (1)

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Show details

Application Notes (2)

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	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Show details
	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Show details

Quick-Start Protocols (2)

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Show details
	(EN) - PyroMark Q24 Vacuum Workstation Quick-Start Guide	Show details

Safety Data Sheets (13)

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q24 Reagents (5 x 24)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Q24 Cartridge (3)
	MSDS PyroMark Q24 Validation Oligo
	MSDS PyroMark Vacuum Prep Filter Probe (100)
	MSDS PyroMark Wash Buffer (conc., 200 ml)

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PyroMark Gold Q96 Reagents (5 x 96)

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UCP Multiplex PCR Kit (100)

For 100 x 20 µl PCR reactions: 1 x 500 µl UCP Multiplex PCR Master Mix (4x), 1 x 200 µl UCP Template Tracer (25x), 1 x 50 µl UCP Master Mix Tracer (125x), 1 x 1.9 ml UCP Water

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PyroMark Q48 Discs (50)

50 discs for automated template preparation and Pyrosequencing reactions using a PyroMark Q48 Autoprep

EUR 152,00

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EpiTect Fast LyseAll Bisulfite Kit (50)

For 50 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers

EUR 236,00

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PyroMark Q48 Advanced CpG Reagents (4 x 48)

Reagents for 4 x 48 PyroMark Q48 Autoprep CpG and long-read reactions: PyroMark Advanced Enzyme Mix, PyroMark Advanced Substrate Mix, Denaturation Solution, Annealing Buffer, Binding Buffer, Nucleotides

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Cat No./ID: 970922

PyroMark Q24 Advanced CpG Reagents (4 x 24)

Enzyme mix, substrate mix, nucleotides, and buffers for CpG and long read Pyrosequencing assays; intended for use with PyroMark Q24 Advanced

EUR 294,00

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EpiTect PCR Control DNA Set (100)

Human control DNA set (containing both bisulfite converted methylated and unmethylated DNA and unconverted unmethylated DNA) for 100 control PCRs

EUR 395,00

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Cat No./ID: 979101

PyroMark Q96 HS Plate (100)

For sample analysis of DNA template on PyroMark Q96 MD

EUR 822,00

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Cat No./ID: 974203

PyroMark Q48 Magnetic Beads (300)

Magnetic streptavidin-coated Sepharose beads for running 300 PyroMark Q48 Autoprep reactions

EUR 123,00

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Cat No./ID: 979004

PyroMark Q96 Cartridge (3)

For delivery of nucleotides and reagents for use with PyroMark Q96 ID

EUR 435,00

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Cat No./ID: 979102

PyroMark Q96 HS Reagent Tip (4)

Reusable tips (4 in each package); for dispensing reagents (RDTs); for use with PyroMark Q96 MD

EUR 310,00

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Cat No./ID: 972812

PyroMark Gold Q96 SQA Reagents (1 x 96)

For performing Pyrosequencing reactions on the PyroMark Q96 ID

EUR 199,00

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Cat No./ID: 979104

PyroMark Q96 HS Capillary Tip (8)

Reusable capillary tips (8 in each package); for dispensing nucleotides (CDTs); for use with PyroMark Q96 MD

EUR 318,00

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QX RNA Alignment Marker (1.5 ml)

RNA alignment marker

EUR 55,40

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Cat No./ID: 979001

PyroMark Q96 Plate (100)

For sample analysis of DNA template prepared with magnetic beads; for use with PyroMark Q96 ID

EUR 233,00

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