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QIAsymphony SP/AS Accessories

For use with the QIAsymphony SP/AS instruments

Features

    Useful Resources

    Important Note — Sample Prep Cartridge positioning in unit boxes Using the KRAS PCR Kit on the Rotor-Gene® Q Instrument - (EN) Using the EGFR PCR Kit on the Rotor-Gene® Q Instrument - (EN)
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    Carrier, tubes (1), sample track, Qsym

    Cat. No. / ID: 9019873

    Tube carrier for sample input; for use with the QIAsymphony SP
     €397.00
    ProductTubeAccessoriesTips
    Carrier, tubes (1), sample track, Qsym
    Adapter 2 x Rotor-Disc, Qsym
    Accessory Trough
    Tube Insert, 11 mm, Revision, sample carrier, Qsym (24)
    Tubes, conical,
    Tube Insert, 13 mm, sample carrier, Qsym (24)
    Tubes, conical
    Cooling Adapter, PCR, v2, Qsym
    Adapter, tubes, 2 ml, v2, Qsym
    Cooling Adapter, EMT, v2, Qsym
    Insert, 2.0ml v2, samplecarr. (24), Qsym
    Cooling Adapter, MTP, RB, v2, Qsym
    Cooling Adapter, Reagent Holder 1, Qsym
    Cooling Adapter, LC capillaries 32, Qsym
    Cooling Adapter, Reagent Holder 2, Qsym
    Cooling Adapter, RG Strip Tubes 72, Qsym
    Cooling Adapter, 2 ml, v2, Qsym
    Cooling Adapter
    8-Rod Covers
    Cooling Adapter, Snap-Cap Microtube QIAsymphony, Qsym
    Filter-Tips, Qsymp SP
    Filter-Tips
    Rotor-Disc 72 Loading Block
    Reagent Cartridge Holder
    Reuse Seal Set
    Set, O-Ring, Qsym
    Tip Disposal Bags (15)
    Sample Carrier, plate, Qsym
    Sample Prep Cartridges
    Add to cart

    Product Details

    QIAsymphony SP/AS Accessories are for use with the QIAsymphony SP/AS instruments for automated sample preparation and assay setup. When ordering adapters, ensure that you order the correct adapter for the software version that you are using.

    Resources

    Kit Handbooks (1)
    Important Note — Sample Prep Cartridge positioning in unit boxes
    PDF (198KB)
    Important information regarding proper positioning of Sample Prep Cartridges within unit boxes
    Supplementary Protocols (2)
    Using the KRAS PCR Kit on the Rotor-Gene® Q Instrument - (EN)
    PDF (255KB)
    Using the EGFR PCR Kit on the Rotor-Gene® Q Instrument - (EN)
    PDF (699KB)
    Brochures & Guides (1)
    (EN) - QIAsymphony RGQ — Pure Performance
    PDF (1MB)
    Labware Files and Documents (1)
    (EN) - QIAsymphony SP/AS adapters and tube inserts
    PDF (1MB)

    FAQ

    Do the master mixes in Rotor-Gene Kits contain dUTP to allow UNG pretreatment?

    No. The master mixes in Rotor-Gene Kits contain dTTP instead of dUTP. If UNG treatment is required, we recommend using QuantiTect +UNG Kits. QuantiTect Kits are also compatible with the Rotor-Gene Q; however, the kits require a significantly longer cycling time.

     

     

    FAQ ID -2117
    If housekeeping and target genes differ significantly in abundance, will they both be amplified with equal efficiency using the Rotor-Gene Multiplex PCR Kit?

    Yes. The optimized master mix of the Rotor-Gene Multiplex PCR Kit ensures that all gene targets in a multiplex reaction are amplified with the same efficiency and sensitivity as in corresponding singleplex reactions, independent of starting copy numbers.

     

    FAQ ID -2129
    Do tissue samples require pretreatment before loading onto the QIAsymphony SP for DNA extraction?
    Yes. Samples have to be completely lysed and homogenized prior to automated processing on the QIAsymphony SP platform using the QIAsymphony DSP DNA Mini Kit. Tissue samples are lysed by proteinase K digestion. FFPE tissue samples are deparaffinized with Deparaffinization Solution or xylene/ethanol and lysed by proteinase K digestion. Insoluble material is pelleted by centrifugation. Lysates are placed on the QIAsymphony SP for purification of DNA (see protocol sheets for details).
    FAQ ID -2926
    Are probes other than TaqMan® probes, such as FRET probes, compatible with fast cycling with the Rotor-Gene Multiplex PCR Kit?

    The Rotor-Gene Multiplex PCR Kit has been developed primarily for use with TaqMan® probes. Protocols for probes other than TaqMan® probes have not been tested.

    See trademarks. 

    FAQ ID -2130
    What types of reaction vessels are required for use in the Rotor-Gene Q?

    We strongly recommend use of the dedicated Rotor-Gene Q Accessories, e.g., PCR Tubes 0.2 ml or Strip Tubes and Caps 0.1 ml provided by QIAGEN. These tubes are designed to give low background and perfectly match the Rotor-Gene Q requirements.

     

    FAQ ID -2085
    How many samples can be run in parallel with the Rotor-Gene Q real-time PCR instrument?

    The Rotor-Gene Q instrument can be used with two different rotor formats: using tubes or rotor-dics. Tubes can be run in 36 and 72-well rotors and rotor-discs in Rotor-Disc 72 and Rotor-Disc 100 rotors. When programming the temperature profile please make sure the correct rotor type is selected.  

     

     

    FAQ ID -1519
    What real-time cycler should I use for my qPCR experiments?
    There are several manufacturers of high-quality real-time cyclers. These include QIAGEN's Rotor-Gene Q, Applied Biosystems, BioRad, Stratagene, Eppendorf, Roche, TaKaRa, Fluidigm, and Cepheid. The important thing to keep in mind is that, once you select an instrument to use, you must use compatible Rotor-Gene Discs and tubes, 96 or 384 well plates, and qPCR master mixes that are optimized for use in that particular instrument.  For example, QIAGEN's Rotor-Gene SYBR Green, Probe, and Multiplex real-time master mixes.  
    FAQ ID -2670
    Are Rotor-Gene Kits compatible with reaction setup using the QIAgility instrument?

    The majority of the Rotor-Gene Kit data shown in our literature has been generated with the help of the QIAgility instrument. We did not observe any problems during the pipetting steps.

     

    FAQ ID -2116
    What are the main differences between Rotor-Gene and QuantiTect or QuantiFast PCR Kits?

    Rotor-Gene Kits are specifically developed for the Rotor-Gene Q PCR Cycler. The unique rotary system of the cycler combined with the kits’ proprietary buffer system enable ultrafast cycling. Rotor-Gene Kits do not contain ROX dye since no normalization to a passive reference is required. Also, Rotor-Gene Kits do not contain dUTP; therefore, UNG pretreatment is not possible.

     

    FAQ ID -2119
    Why have the hazard symbols accompanying products changed?
    The Globally Harmonized System (GHS) is an initiative by the United Nations aiming to harmonize chemical hazard communication worldwide. Hazards symbols accompanying products are changing to those of the GHS. Information on the adoption of this new system is available on our GHS Hazard Labeling pages.
    FAQ ID - 3430

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