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GeneRead Size Selection Kit

For quick and reliable removal of DNA fragments <150 bp for library preparation in NGS applications

Features

  • Precise size selection of DNA fragments
  • Fast procedure, based on QIAGEN’s proven silica-column technology
  • An easy-to-follow protocol, automatable on the QIAcube
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GeneRead Size Selection Kit (50)

Cat. No. / ID: 180514

For 50 reactions: Spin columns and buffers
€130.00
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The GeneRead Size Selection Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The GeneRead Size Selection Kit enables precise size selection and purification of DNA fragments using a fast and convenient spin-column-based procedure. The kit contains MinElute columns and an optimized binding buffer for the selection of DNA fragments larger than 150 bp. Procedures can be automated on the QIAcube Connect.

Performance

Fast and precise size selection
The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers (see figure  Precise size selection). DNA fragments shorter than 150 bp are reliably removed. 

See figures

Principle

The GeneRead Size Selection Kit combines the convenience of spin-column technology with the selective properties of a uniquely designed binding buffer. MinElute spin columns provided with the kit ensure high concentrations of purified DNA for subsequent reactions. The special buffer provided with the kit is optimized for efficient removal of small DNA fragments such as adapters and adapter dimers. Larger DNA fragments adsorb to the silica membrane in the presence of optimized concentrations of salt, while contaminants and smaller fragments pass through the column. This leads to a cut-off limit (defined as the length of the shortest fragment that can be visualized by electropherogram) of 150 bp. Impurities such as enzymes from previous reactions are efficiently washed away, and  pure and size-selected DNA is eluted. For optimal adapter monomer and dimer removal, together with high DNA recovery, two subsequent purification steps are performed for size selection during library preparations for next-generation sequencing.

Procedure

The GeneRead Size Selection Kit uses a convenient, spin-column-based procedure to ensure precise size selection of the DNA library, while effectively removing adapter dimers or monomers. Automatable on the QIAcube, the easy-to-use protocol saves time by eliminating tedious handling procedures, and ensures there is no risk of carryover of potentially inhibitory ethanol or beads (see figure  Precise size selection).
See figures

Applications

The GeneRead Size Selection Kit enables quick and reliable removal of DNA fragments shorter than 150 bp for subsequent use in library preparation in NGS.

Supporting data and figures

Resources

Cleanup (1)
Protocol Sheet for QIAcube Classic - GeneRead Size Selection - sheared DNA protocol
Brochures & Guides (2)
Introducing QIAseq
PDF (450KB)
Accelerate your NGS performance through Sample to Insight solutions
Accelerate your NGS performance through Sample to Insight solutions
Quick-Start Protocols (1)
Additional Resources (1)
For the preparation, size selection, and purification of DNA libraries for NGS applications
Protocol Files (1)
Cleanup of sheared DNA and size selection

This protocol is for cleanup and reliable removal of DNA fragments <150 bp for library preparation in NGS applications.

Sample Size 140 µL
Elution volume 17 µL
Applications Cleanup
Starting material DNA
Kit Handbooks (2)
For fast and reliable removal of DNA fragments <150 bp for library preparation in next-generation sequencing (NGS)
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.

FAQ

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
3204 - How strict is the purification cut-off with GeneRead Size Selection kit?

Full recovery is seen for fragments larger than 300bp. Between 150 and 300bp, recovery increases with size.

Buffer SB1 in the GeneRead Size Selection Kit turned yellowish from a new kit. Can I still use it?

It is normal for the buffer to turn yellow and it does not impact the performance of the buffer.

FAQ ID - 3562