miScript miRNA Mimics

For studies on miRNA function and gene regulation using synthetic miRNA

Features

  • Ready-to-transfect mimics behave like endogenous miRNAs
  • Available for all human, mouse, and rat miRNAs in miRBase
  • Highly flexible formats, scales, and grades
  • Easy to order via GeneGlobe
miScript miRNA Mimic

Cat. No. / ID: 219600

1 nmol (cell-culture grade), 5 nmol (cell-culture grade), or 20 nmol (animal grade) miScript miRNA Mimic, provided in tubes
 CONFIGURE AT GeneGlobe
miScript miRNA Mimics are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

miScript miRNA Mimics are chemically synthesized, double-stranded RNAs which mimic mature endogenous miRNAs after transfection into cells. miScript miRNA Mimics are available at cell-culture grade (>90% purity) or animal grade (HPLC purified; for in vivo applications). For flexible screening, miScript miRNA Mimic Plates enable researchers to order any predesigned miScript miRNA Mimics in their choice of layout in 96-well or 384-well plates. miScript miRNA Inhibitors are suitable for similar experiments. For transfection, QIAGEN provides HiPerFect Transfection Reagent for miRNA Mimic transfection and Attractene Transfection Reagent for miRNA Mimic-DNA cotransfection.

For even higher performance in your miRNA studies, try our miRCURY LNA miRNA Mimics. These highly potent, mature miRNA mimics feature a unique, triple RNA strand design for superior results.

Performance

miScript miRNA Mimics cause a decrease in gene expression similar to that observed with endogenous miRNAs (see figure " Comparable downregulation by endogenous miRNA and miScript miRNA Mimic"). The observed decrease in gene expression is often mediated at the translational level (see figure " Downregulation at protein level after miR-1 Mimic transfection"). miScript miRNA Mimics efficiently inhibit gene expression for up to 72 hours after transfection (see figure " miScript miRNA Mimic stable for 72 hours").
See figures

Principle

Transfection of mimics followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs. Reduced gene expression after transfection of mimic provides evidence that the miRNA under study is involved in regulation of that gene. miScript miRNA Inhibitors can also be used for similar studies. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.

Procedure

miScript miRNA Mimics are available for every human, mouse, rat, and virus miRNA in the current version of miRBase, the microRNA database. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. 

Flexible formats enable either low-throughput analysis of small numbers of mimics or high-throughput screening of hundreds of mimics. miScript miRNA Mimics are available in tubes (1 nmol or 5 nmol) at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Mimics can be ordered at the 20 nmol scale (provided in tubes). For medium- to high-throughput applications, 96-well plates (1 nmol or 5 nmol), or 384-well plates (1 nmol) are available to suit your experimental requirements. 

Applications

  • miRNA research
  • Gene regulation research

Supporting data and figures

Specifications

FeaturesSpecifications
designPredesigned and custom design avaliable
speciesHuman, mouse, rat
formatSingle tubes, 96-well & 384 well plates
scale1 nmol, 5 nmol, 20 nmol
modificationNone
sequenceprovidedNo

Resources

Brochures & Guides (2)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
miRNA Research (1)

FAQ

What is the turn around time for custom miScript miRNA mimics, inhibitors and primer assays?

The turn around time for custom miScript miRNA Mimics, Inhibitors and Primer Assays is 2-3 business days, plus 2 days for shipping.

 

FAQ ID -1985
What kind of molecules are miScript miRNA Mimics?

miScript miRNA Mimics are synthetic double stranded, blunt end, RNA oligos.

 

FAQ ID -1986
Where can I find QIAGEN products for a specific gene or gene product?
You can search for specific gene products in the QIAGEN GeneGlobe Database. This easy-to-use, comprehensive Web portal allows you to find information about, search for, and order high-quality products for human, mouse, and rat genes. QIAGEN provides a vast range of gene-specific products covering every aspect of an experiment, from gene silencing to expression analysis at the mRNA or protein level.
FAQ ID -803
Does HiPerFect work for the transfection of miScript miRNA mimics and inhibitors?

HiPerFect Transfection Reagent does work for the transfection of miScript miRNA Mimics and Inhibitors, and the new handbook contains protocols for this application.

 

 

FAQ ID -1984
What is the "seed sequence" of a miRNA?
miRNAs regulate the gene expression by binding to the mRNA. The seed sequence is essential for the binding of the miRNA to the mRNA. The seed sequence or seed region is a conserved heptametrical sequence which is mostly situated at positions 2-7 from the miRNA 5´-end. Even though base pairing of miRNA and its target mRNA does not match perfect, the “seed sequence” has to be perfectly complementary.
FAQ ID -2262