text.skipToContent text.skipToNavigation

QIAseq FastSelect RNA Removal Kits

For rapid rRNA and/or globin mRNA removal for RNA-seq library preparation
  • Compatible with QIAGEN, Illumina, NEB and KAPA RNA stranded library kits
  • High performance rRNA and/or globin removal in just 20 minutes
  • Only one pipetting step – combine removal reagent with RNA and incubate
  • No extra cleanup steps or protocol modifications
  • Customize to your experiments by species and targets

NEW!  QIAseq FastSelect –rRNA HMR Kits for rRNA and/or globin mRNA removal are 30% faster and more powerful than our previous version. Click here to view!

 

QIAseq FastSelect RNA Removal Kits use a novel method to remove highly abundant RNA that is of low scientific value from your RNA-seq libraries. Whether you are performing whole transcriptome analysis and want to remove cytoplasmic and mitochondrial rRNA or doing gene expression research from blood samples and require globin mRNA removal, QIAseq FastSelect Kits specifically remove the targeted RNA in a single pipetting step, without additional protocol changes or cleanup steps. 
Also look at QIAseq Stranded RNA Library Kits for robust RNA-seq library preparation.

 

Interested in customization of a QIAseq FastSelect RNA Removal Kit for your samples? Contact us!

Buy Products

Cat No./ID: 333180
QIAseq FastSelect RNA Removal Kit
Go to GeneGlobe
Reagents for rRNA removal or globin mRNA removal for 24, 96 or 384 samples; human, mouse or rat
Cat No./ID: 333280
QIAseq FastSelect Multi-RNA Removal Kit
Go to GeneGlobe
Reagents for rRNA and globin mRNA removal for 24, 96 or 384 samples; human, mouse or rat

Product Details

Performance
QIAseq FastSelect RNA Removal Kits result in highly efficient removal of rRNA to levels observed with Poly-A enrichment. Using sequencing quality control samples (SEQC), QIAseq FastSelect demonstrated high performance rRNA removal and highly reproducible gene expression results.
See Figures:
QIAseq FastSelect vs. no treatment, Poly-A enrichment and Ribo-Zero Gold (% rRNA remaining)
QIAseq FastSelect vs. no treatment, Poly-A enrichment and Ribo-Zero Gold (gene expression results)
QIAseq FastSelect performance with sequencing quality control (SEQC) samples (% rRNA remaining)
QIAseq FastSelect performance with sequencing quality control (SEQC) samples (gene expression results)
QIAseq FastSelect performance with sequencing quality control (SEQC) samples (principal component analysis)
QIAseq FastSelect performance with sequencing quality control (SEQC) samples (Venn diagram)

Robust performance with a variety of sample types


QIAseq FastSelect provides reliable rRNA removal and reproducible gene expression results with RNA extracted from various sample types, including FFPE, human tissues and cell lines.
See Figures:
QIAseq Fast Select shows robust performance with RNA from FFPE samples (% rRNA remaining)
Highly reproducible gene expression data following rRNA depletion for RNA from FFPE samples
QIAseq FastSelect provides robust performance with FFPE standards (% rRNA remaining)
QIAseq FastSelect provides robust performance with FFPE standards (gene expression results)
QIAseq FastSelect provides robust performance with RNA from tissue samples (% rRNA remaining)
QIAseq FastSelect provides robust performance with RNA from tissue samples (gene expression results)
QIAseq FastSelect provides robust performance with RNA from cell lines (% rRNA remaining)
QIAseq FastSelect provides robust performance with RNA from cell lines (gene expression results)

Integrates with existing stranded library construction workflows


Requiring just a single, inline pipetting step, QIAseq FastSelect is fully compatible with Illumina, NEB and KAPA RNA stranded library kits.
See Figures:
QIAseq FastSelect integrated into Illumina TruSeq workflow (performance)
QIAseq FastSelect integrated into Illumina TruSeq workflow (gene expression results)
QIAseq FastSelect integrated into NEBNext Ultra II Directional RNA Library workflow (performance)
QIAseq FastSelect integrated into NEBNext Ultra II Directional RNA Library workflow (gene expression results)
QIAseq FastSelect integrated into KAPA RNA HyperPrep workflow (performance)
QIAseq FastSelect integrated into KAPA RNA HyperPrep workflow (gene expression results)


Superior performance, faster workflow


QIAseq FastSelect is considerably faster than other methods and provides superior performance.
See Figures:
QIAseq Fast Select shows robust performance with RNA from FFPE samples (% rRNA remaining)
Highly reproducible gene expression data following rRNA depletion for RNA from FFPE samples
QIAseq FastSelect provides robust performance with FFPE standards (% rRNA remaining)
QIAseq FastSelect provides robust performance with FFPE standards (gene expression results)
QIAseq FastSelect provides robust lot-to-lot reproducibility for RNA depletion (% rRNA remaining)
QIAseq FastSelect provides robust lot-to-lot reproducibility for RNA depletion (gene expression results)
Principle
Removing highly expressed, but biologically unimportant RNA transcripts makes NGS more efficient and enables higher sample throughput with higher sensitivity. Furthermore, RNA from FFPE samples and degraded RNA samples are particularly challenging for RNA removal and can result in suboptimal performance.

QIAseq FastSelect is designed for quick, efficient removal of cytoplasmic and mitochondrial rRNA and/or globin mRNA from total RNA during NGS RNA library preparation. QIAseq FastSelect seamlessly integrates with your existing RNA stranded library preparation workflow for RNA removal in a single, 20-minute inline step. Prior to RNA heat fragmentation (which is optional and dependent upon the library preparation kit and sample type), QIAseq FastSelect removal reagent is directly combined with total RNA and the library preparation-specific buffers. After fragmentation, the reaction temperature is step-wise cooled to room temperature and the remaining library preparation steps are completed. There is no need to perform any type of enrichment on the total RNA samples. QIAseq FastSelect RNA Removal Kits can accommodate RNA amounts ranging from as little as 1 ng up to 1 µg with consistently high performance. QIAseq FastSelect can be used with RNA from fresh samples, as well as RNA from FFPE (formalin-fixed paraffin-embedded) samples or degraded RNA, resulting in reliable rRNA removal and high reproducibility in downstream applications.
Procedure
Most RNA removal or depletion strategies associated with RNA-seq library construction are sample pre-treatment strategies involving hybrid-capture or enzymatic removal of unwanted RNA. Our unique QIAseq FastSelect procedure is compatible with QIAGEN, Illumina, KAPA and NEB stranded library preparation kits and provides complete rRNA removal in a single, 20-minute inline step (Figure QIAseq FastSelect RNA removal workflow). This is dramatically faster than competing RNA depletion kits, which require pre-treatment protocols involving more than 25 steps and 2 hours to complete.

Simply add QIAseq FastSelect reagent (rRNA Removal and/or Globin Removal) to the RNA sample, perform fragmentation (if required), stepwise cool the reaction from 75°C to 25°C for 20 minutes and then complete the remaining library preparation steps. QIAseq FastSelect works with or without RNA fragmentation, providing the flexibility to use RNA from FFPE samples or degraded RNA samples, or high-quality RNA as part of a standard RNA-seq library construction workflow.
Applications
QIAseq FastSelect delivers rapid, reliable RNA removal for FFPE and fresh sample RNA sources. QIAseq FastSelect RNA Removal Kits are available in a variety of different formats and sizes to suit your specific applications. QIAGEN offers kits for removing human, mouse and rat RNA, as well as a customizable product (coming soon) designed for the species and RNA of your choice.

Wide array of QIAseq FastSelect products
Product Species available Targets Number of samples
QIAseq FastSelect RNA Removal Kit Human, mouse and rat rRNA or globin mRNA 24, 96 or 384
QIAseq FastSelect Multi-RNA Removal Kit Human, mouse and rat rRNA and globin mRNA 24, 96 or 384
QIAseq FastSelect Custom RNA Removal Kit (coming soon) Your choice Any RNA 1156

Product Resources

fragment fix placeholder

Customers who bought these products also bought