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QIAamp PowerFecal Pro DNA Kit

For the isolation of microbial DNA from stool and gut samples
  • Efficient lysis of bacteria and fungi from stool and gut samples
  • Up to 20-fold more DNA compared to alternative methods
  • Yield higher alpha diversity in sequencing compared to other methods
  • Recovery of inhibitor-free DNA for direct use in next-generation sequencing applications
  • Automate workflows on the QIAcube

The QIAamp PowerFecal DNA Kit removes tough inhibitors from stool, gut and biosolid samples and provides clean, ready-to-use DNA for any downstream application. With the included Inhibitor Removal Technology, inhibitors from digested food, heme from lysed red blood cells in stool and other PCR inhibitors are eliminated leaving only high-quality DNA ready for downstream applications such as qPCR, Sanger sequencing and next-generation sequencing.


The QIAamp PowerFecal DNA Kit can be used interchangeably with the DNeasy PowerSoil Kit. Both kits can be used to isolate DNA from soil, environmental, stool, gut and biosolid samples.


Isolation of DNA using the QIAamp PowerFecal DNA Kit can be automated on the QIAcube Connect. Want to try this solution for the first time? Request a quote for a trial kit.

QIAamp PowerFecal DNA Kit was formerly sold by MO BIO as PowerFecal DNA Isolation Kit.

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Cat No./ID: 12830-50
QIAamp PowerFecal DNA Kit
EUR 354,00
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For the isolation of DNA from stool, gut material and biosolids
Cat No./ID: 12830-S
QIAamp PowerFecal DNA Sample (2)
Isolate DNA from stool, gut material and biosolids.
Cat No./ID: 51804
QIAamp PowerFecal Pro DNA Kit
EUR 409,00
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For the isolation of microbial DNA from stool and gut samples
The QIAamp PowerFecal Pro DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

0
Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.
DNA was isolated from stool samples (200 mg) from three donors using commercially available sample preparation solutions and the new QIAamp PowerFecal Pro DNA Kit and compared. Yields were measured by fluorometric quantification (Qubit™).
1
The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high-quality DNA.
A DNA from duplicate 1.8 ml C. albicans cultures was isolated with the QIAamp PowerFecal DNA (A) or the QIAamp PowerFecal Pro DNA (B) Kits. B, C DNA from stool samples (200 mg) from three donors was prepared with the first-generation QIAamp PowerFecal DNA Kit B or the new QIAamp PowerFecal Pro DNA Kit C. DNA was analyzed via gel electrophoresis.
2
Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.
Stool samples (200 mg) were prepared using commercially available sample preparation solutions or the new QIAamp PowerFecal Pro DNA Kit and compared.
DNA purity was measured via UV spectrophotometry and the comparisons of A260/A280 A and A260/A230 B ratios are shown..
3
Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.
DNA prepared from stool samples was isolated with different methods and enriched. Analysis of the 16S rRNA genes was done using the QIAseq® 1-Step Amplicon Kit and data analysis was done using the Microbial Genomics Pro DNA Suite (CLC workbench). Alpha diversity was determined by total number of bacterial OTUs.
Performance

Using improved technologies, the new QIAamp PowerFecal Pro Kit allows researchers to obtain higher yields of DNA from stool and gut samples compared with alternative methods (See: ‘Higher yields of DNA with the QIAamp PowerFecal Pro DNA Kit.’).

Improved yields of higher quality DNA are also achieved when comparing the QIAamp PowerFecal Pro DNA Kit to the first generation QIAamp PowerFecal DNA Kit when running stool and fungal samples (See: ‘The new QIAamp PowerFecal Pro DNA Kit enables the isolation of larger amounts of high quality DNA.’).

Principle
The streamlined Inhibitor Removal Technology® (IRT) used by the new QIAamp PowerFecal Pro DNA Kit is highly efficient at removing inhibitors while still decreasing sample processing time. The QIAamp PowerFecal Pro DNA Kits also showed the highest A260/A280 ratios, near 1.8, of any other commercially available kit, indicating the absence of inhibitors. Less variability was also observed between the samples processed with QIAamp PowerFecal Pro DNA Kits (See: ‘Highly pure DNA isolated using the new QIAamp PowerFecal Pro DNA Kit.’). Researchers using the QIAamp PowerFecal Pro DNA Kit can identify a greater bacterial diversity with increased OTUs from stool samples compared with the first-generation QIAamp PowerFecal Kit and other commercially available kits (See: ‘Greater total number of bacterial OTUs isolated with the new QIAamp PowerFecal Pro DNA Kit.’).
Procedure
Samples processed with the QIAamp PowerFecal Pro DNA Kit are added to a bead beating tube. Rapid and thorough homogenization occurs using mechanical and chemical methods. Once cells are lysed, our patented IRT is used to remove inhibitors. Total genomic DNA is captured on a silica membrane in a spin-column format. DNA is then washed and eluted, ready for downstream applications.
Applications

Tools to complete your workflow:


  1. Sample disruption: PowerLyzer 24 Homogenizer
  2. Sample extraction: QIAamp PowerFecal DNA Kit
  3. Library preparation: QIAseq 1-step Amplicon Kit (16S rRNA gene sequencing) or QIAseq FX DNA Library Kit (metagenomic shotgun sequencing)
  4. Analysis: QIAGEN Microbial Genomics Pro Suite (request a demo)


Specifications

Features
Specifications
Bead size 0.7 mm garnet
Binding capacity Up to 20 µg per filter
Format Silica spin filter tubes
Processing Bead beating
Sample size 0.25 g
Sample types Stool, gut material, biosolids, soil
Storage temperature Store at room temperature (15-30°C)
Throughput 1-24 samples
Time per run or per prep 30 minutes

Product Resources

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Kit Handbooks (1)
For the isolation of microbial genomic DNA from stool and gut samples
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Webinars (1)
This webinar will focus on novel techniques for obtaining unbiased results in 16S rRNA gene sequencing of challenging microbiome samples.
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