QuantiFERON Monitor (QFM) test

For detection of cell mediated immune response in immunosuppressed solid organ transplant patients

Features

  • Assesses cell-mediated immune (CMI) response
  • Provides nonpathogen specific immune monitoring
  • Complements pathogen specific monitoring with QuantiFERON-CMV test
  • Aids in clinical management of solid organ transplant patients

Products

QuantiFERON Monitor is intended for use in conjunction with risk assessment and other medical and diagnostic evaluations. Reference QuantiFERON Monitor (QFM) ELISA Package Insert. Rev. 01. November 2014.
The QuantiFERON Monitor (QFM) test is intended for in vitro diagnostic use.
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QuantiFERON Monitor (QFM) Blood Collection Tubes (100)

Cat. No. / ID: 0650-0101

For drawing blood for use with the QFM assay, 100 each
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QFM 2-Plate Kit ELISA

Cat. No. / ID: 0650-0201

QuantiFERON Monitor 2-plate kit includes: Microplate strips; Human IFN-γ Standard, lyophilized; Green Diluent; Conjugate 100x Concentrate, lyophilized; Wash Buffer 20x Concentrate; Enzyme Substrate Solution; Enzyme Stopping Solution
€1,314.00
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QFM LyoSpheres

Cat. No. / ID: 0650-0701

QuantiFERON Monitor LyoSpheres include: 10 vials of pelleted lyophilized stimulants (1 pellet per vial)

Product Details

QuantiFERON Monitor (QFM) test is an innovative in vitro diagnostic test that detects interferon γ (IFNγ) following incubation of heparinized whole blood with innate and adaptive immune response stimulants.

Performance

Use QFM to assess cell-mediated immune response in the immunosuppressed solid organ transplant population.

Principle

After incubation of heparinized whole blood with innate and adaptive immune response stimulants (Toll Like Receptor and T cell receptor agonists, respectively) QFM test detects cell-mediated immune function through the measurement of interferon gamma (IFNγ) in plasma by enzyme-linked immunosorbent assay.

Procedure

The QFM test is performed in stages:
  1. Collect whole blood into the QFM Blood Collection Tube.
  2. Add a QFM LyoSphere to the QFM Blood Collection Tube, and incubate the tube.
  3. After incubation, centrifuge the tube to isolate plasma.
  4. Measure IFNγ in the extracted plasma by ELISA and compare the responses to a range of expected values to characterize immune response of the subject.

Applications

QuantiFERON Monitor test may be used to detect cell-mediated immune response in immunosuppressed patients who have undergone solid organ transplantation. QFM test is complementary to QuantiFERON-CMV test in this population for posttransplant patient management.

Software

Software is available at www.QuantiFERON.com.

Resources